ID   C71B3_ARATH             Reviewed;         501 AA.
AC   O65785; Q8W4B9; Q9LTL9;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Cytochrome P450 71B3;
DE            EC=1.14.-.-;
GN   Name=CYP71B3; OrderedLocusNames=At3g26220; ORFNames=MTC11.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Seedling;
RX   PubMed=9620263; DOI=10.1023/a:1005921406884;
RA   Mizutani M., Ward E., Ohta D.;
RT   "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT   differential expression, and RFLP mapping of multiple cytochromes P450.";
RL   Plant Mol. Biol. 37:39-52(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-501.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL32750.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; D78602; BAA28534.1; -; mRNA.
DR   EMBL; AB024038; BAB02443.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77135.1; -; Genomic_DNA.
DR   EMBL; AY062672; AAL32750.1; ALT_INIT; mRNA.
DR   PIR; T52170; T52170.
DR   RefSeq; NP_189253.1; NM_113529.3.
DR   AlphaFoldDB; O65785; -.
DR   SMR; O65785; -.
DR   STRING; 3702.AT3G26220.1; -.
DR   PaxDb; O65785; -.
DR   PRIDE; O65785; -.
DR   ProteomicsDB; 239198; -.
DR   EnsemblPlants; AT3G26220.1; AT3G26220.1; AT3G26220.
DR   GeneID; 822223; -.
DR   Gramene; AT3G26220.1; AT3G26220.1; AT3G26220.
DR   KEGG; ath:AT3G26220; -.
DR   Araport; AT3G26220; -.
DR   TAIR; locus:2093546; AT3G26220.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_1_1; -.
DR   InParanoid; O65785; -.
DR   OMA; MSWFTIC; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; O65785; -.
DR   BioCyc; ARA:AT3G26220-MON; -.
DR   PRO; PR:O65785; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; O65785; baseline and differential.
DR   Genevisible; O65785; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..501
FT                   /note="Cytochrome P450 71B3"
FT                   /id="PRO_0000052081"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         445
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        79..82
FT                   /note="KEAA -> QETT (in Ref. 1; BAA28534)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   501 AA;  57663 MW;  B4A48E39166FBEC5 CRC64;
     MSILLYFFFL PVILSLIFMK KFKDSKRNLP PSPPKLPIIG NLHQLRGLFH RCLHDLSKKH
     GPVLLLRLGF IDMVVISSKE AAEEVLKVHD LECCTRPKTN ASSKFSRDGK DIAFAPYGEV
     SRELRKLSLI NFFSTQKVRS FRYIREEEND LMVKKLKESA KKKNTVDLSQ TLFYLVGSII
     FRATFGQRLD QNKHVNKEKI EELMFEVQKV GSLSSSDIFP AGVGWFMDFV SGRHKTLHKV
     FVEVDTLLNH VIDGHLKNPE DKTNQDRPDI IDSILETIYK QEQDESFKLT IDHLKGIIQN
     IYLAGVDTSA ITMIWAMAEL VKNPRVMKKA QEEIRTCIGI KQKERIEEED VDKLQYLKLV
     IKETLRLHPP APLLLPRETM ADIKIQGYDI PRKTILLVNA WSIGRNPELW ENPEEFNPER
     FIDCPMDYKG NSFEMLPFGS GRKICPGIAF GIATVELGLL NLLYYFDWRL AEEDKDIDME
     EAGDATIVKK VPLELVPIIH H