ID   C71B4_ARATH             Reviewed;         504 AA.
AC   O65786; Q9LTL1;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2004, sequence version 2.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Cytochrome P450 71B4;
DE            EC=1.14.-.-;
GN   Name=CYP71B4; OrderedLocusNames=At3g26280; ORFNames=MTC11.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Seedling;
RX   PubMed=9620263; DOI=10.1023/a:1005921406884;
RA   Mizutani M., Ward E., Ohta D.;
RT   "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT   differential expression, and RFLP mapping of multiple cytochromes P450.";
RL   Plant Mol. Biol. 37:39-52(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; D78603; BAA28535.1; -; mRNA.
DR   EMBL; AB024038; BAB02451.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77141.1; -; Genomic_DNA.
DR   EMBL; AY090254; AAL90915.1; -; mRNA.
DR   EMBL; AY149951; AAN31105.1; -; mRNA.
DR   PIR; T52171; T52171.
DR   RefSeq; NP_189259.1; NM_113535.2.
DR   AlphaFoldDB; O65786; -.
DR   SMR; O65786; -.
DR   BioGRID; 7562; 6.
DR   IntAct; O65786; 6.
DR   STRING; 3702.AT3G26280.1; -.
DR   PaxDb; O65786; -.
DR   PRIDE; O65786; -.
DR   ProteomicsDB; 240494; -.
DR   EnsemblPlants; AT3G26280.1; AT3G26280.1; AT3G26280.
DR   GeneID; 822231; -.
DR   Gramene; AT3G26280.1; AT3G26280.1; AT3G26280.
DR   KEGG; ath:AT3G26280; -.
DR   Araport; AT3G26280; -.
DR   TAIR; locus:2093536; AT3G26280.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_1_1; -.
DR   InParanoid; O65786; -.
DR   OMA; SAIIMIW; -.
DR   PhylomeDB; O65786; -.
DR   PRO; PR:O65786; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; O65786; baseline and differential.
DR   Genevisible; O65786; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..504
FT                   /note="Cytochrome P450 71B4"
FT                   /id="PRO_0000052082"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         446
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        434
FT                   /note="F -> C (in Ref. 1; BAA28535)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   504 AA;  57789 MW;  93451C02A5E96976 CRC64;
     MVSLLSFFLL LLVPIFFLLI FTKKIKESKQ NLPPGPAKLP IIGNLHQLQG LLHKCLHDLS
     KKHGPVMHLR LGFAPMVVIS SSEAAEEALK THDLECCSRP ITMASRVFSR NGKDIGFGVY
     GDEWRELRKL SVREFFSVKK VQSFKYIREE ENDLMIKKLK ELASKQSPVD LSKILFGLTA
     SIIFRTAFGQ SFFDNKHVDQ ESIKELMFES LSNMTFRFSD FFPTAGLKWF IGFVSGQHKR
     LYNVFNRVDT FFNHIVDDHH SKKATQDRPD MVDAILDMID NEQQYASFKL TVDHLKGVLS
     NIYHAGIDTS AITLIWAMAE LVRNPRVMKK AQDEIRTCIG IKQEGRIMEE DLDKLQYLKL
     VVKETLRLHP AAPLLLPRET MADIKIQGYD IPQKRALLVN AWSIGRDPES WKNPEEFNPE
     RFIDCPVDYK GHSFELLPFG SGRRICPGIA MAIATIELGL LNLLYFFDWN MPEKKKDMDM
     EEAGDLTVDK KVPLELLPVI RISL