C71B5_ARATH
ID C71B5_ARATH Reviewed; 498 AA.
AC O65784;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Cytochrome P450 71B5;
DE EC=1.14.-.-;
GN Name=CYP71B5; OrderedLocusNames=At3g53280; ORFNames=T4D2.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=9620263; DOI=10.1023/a:1005921406884;
RA Mizutani M., Ward E., Ohta D.;
RT "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT differential expression, and RFLP mapping of multiple cytochromes P450.";
RL Plant Mol. Biol. 37:39-52(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; D78601; BAA28533.1; -; mRNA.
DR EMBL; AL132958; CAB64231.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79062.1; -; Genomic_DNA.
DR PIR; T46174; T46174.
DR RefSeq; NP_190896.1; NM_115188.5.
DR AlphaFoldDB; O65784; -.
DR SMR; O65784; -.
DR STRING; 3702.AT3G53280.1; -.
DR iPTMnet; O65784; -.
DR PaxDb; O65784; -.
DR PRIDE; O65784; -.
DR ProteomicsDB; 240387; -.
DR EnsemblPlants; AT3G53280.1; AT3G53280.1; AT3G53280.
DR GeneID; 824495; -.
DR Gramene; AT3G53280.1; AT3G53280.1; AT3G53280.
DR KEGG; ath:AT3G53280; -.
DR Araport; AT3G53280; -.
DR TAIR; locus:2102003; AT3G53280.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; O65784; -.
DR OMA; GSITMIW; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; O65784; -.
DR BioCyc; ARA:AT3G53280-MON; -.
DR PRO; PR:O65784; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O65784; baseline and differential.
DR Genevisible; O65784; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..498
FT /note="Cytochrome P450 71B5"
FT /id="PRO_0000052083"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 439
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 498 AA; 56374 MW; 88AC93527AE69014 CRC64;
MSIFLCFLLL LPLSLIFLKK LLPSKGKLPP GPKGLPIIGN LHQFGRFLHK SLHKISQEYG
PVMLLHFGVV PVIIVSSKEG AEEVLKTHDL ETCSRPKTVG SGLFTYNFKD IGFAPYGENW
REMRKIAVSE LFSQKKLKSF RYIREDESQL LVRKVSKSAL ETPTSSVNLR KVIFTFAASI
ICRLSFGQNF CDFVDMETVE ELVLESETNL GSLAFADFLP AGWIIDRISG QHSTVMKAFS
KLTNFFELVI DDHLKSGKIE DHSDIISVML DMINKPTEVG SYKVTDDHLK GLMSDVFLAG
VNAGSITMIW TMTELSRHPR VMRKLQEEIR AALGPNKEKI TEEDLEKVEY LKMVIEEAFR
LHPPAPLLLP RLTMSDINIQ GYSIPKNTMI QINTYTIGRD PKNWTKPDEF IPERFVDNPI
EYKGQHFELL PFGAGRRVCP GMATGITIVE LGLLSLLYFF DWSLPNGMTT KDIDMEEDGA
FVIAKKVSLE LVPTLHRW
