ID   C71B6_ARATH             Reviewed;         503 AA.
AC   O65787;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Cytochrome P450 71B6;
DE            EC=1.14.-.-;
GN   Name=CYP71B6; OrderedLocusNames=At2g24180; ORFNames=F27D4.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Seedling;
RX   PubMed=9620263; DOI=10.1023/a:1005921406884;
RA   Mizutani M., Ward E., Ohta D.;
RT   "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT   differential expression, and RFLP mapping of multiple cytochromes P450.";
RL   Plant Mol. Biol. 37:39-52(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; D78604; BAA28536.1; -; mRNA.
DR   EMBL; AC005967; AAD03379.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07536.1; -; Genomic_DNA.
DR   EMBL; AY054534; AAK96725.1; -; mRNA.
DR   EMBL; AY064632; AAL47345.1; -; mRNA.
DR   PIR; T52172; T52172.
DR   RefSeq; NP_179995.1; NM_127979.3.
DR   AlphaFoldDB; O65787; -.
DR   SMR; O65787; -.
DR   BioGRID; 2304; 18.
DR   IntAct; O65787; 19.
DR   STRING; 3702.AT2G24180.1; -.
DR   PaxDb; O65787; -.
DR   PRIDE; O65787; -.
DR   ProteomicsDB; 240495; -.
DR   EnsemblPlants; AT2G24180.1; AT2G24180.1; AT2G24180.
DR   GeneID; 816952; -.
DR   Gramene; AT2G24180.1; AT2G24180.1; AT2G24180.
DR   KEGG; ath:AT2G24180; -.
DR   Araport; AT2G24180; -.
DR   TAIR; locus:2047570; AT2G24180.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_1_1; -.
DR   InParanoid; O65787; -.
DR   OMA; HIRAIIM; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; O65787; -.
DR   BioCyc; ARA:AT2G24180-MON; -.
DR   PRO; PR:O65787; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O65787; baseline and differential.
DR   Genevisible; O65787; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0005773; C:vacuole; HDA:TAIR.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IDA:TAIR.
DR   GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   GO; GO:0042430; P:indole-containing compound metabolic process; IDA:TAIR.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..503
FT                   /note="Cytochrome P450 71B6"
FT                   /id="PRO_0000052084"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         446
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   503 AA;  57009 MW;  71AFAD5370AEDFF6 CRC64;
     MSLFSFPIST ELLPWLLLLL IPPLLIFFLL RSPKNLPPGP PRLPILGNIH QLGSLPHRSL
     RDLSLKYGPV ITVYLGSVRT VVVHSPETAE EVLKLHDSEC CTRPKLSITK SFFYDGLGLG
     FTKWGDYYRD VRKLCVLELF SVKRANSFRN IREEELSRLV NSFSDSASSG SSVDLTANLA
     KFVASFTCRM AFGLSFQGSG MDNETFLELF TEANRVIGKF AAADIFPGFG WILDRISGLD
     SSRRKSFQDL DTFYQKAIVD HREKKKTEDR EDLIDVLLKL QSQETKLGSS RITDTHIRAI
     IMDLFVAGVD TSVITLDWTM AELSRHPRVM KKVQAEIREH VGDKGIVTYD DLEALVYMKM
     VIKETWRLHA PSPILIPREA MTNFKIKGYD IYPGTRIHVN AWAIGRNPDV WKDPDEFIPE
     RFVDSNVETK GTSFELLPFG SGRRGCPAMY VGLSTVEYTL ANLLYHFDWK ATEEVSVEEA
     PGLTSHRKHP LHLVPVNVIN RKL