ID   C71B9_ARATH             Reviewed;         500 AA.
AC   O64718; Q8VYE9;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 3.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=Cytochrome P450 71B9;
DE            EC=1.14.-.-;
GN   Name=CYP71B9; OrderedLocusNames=At2g02580; ORFNames=T8K22.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AC004136; AAC18928.2; -; Genomic_DNA.
DR   EMBL; CP002685; AEC05601.1; -; Genomic_DNA.
DR   EMBL; AY072124; AAL59946.1; -; mRNA.
DR   PIR; T00605; T00605.
DR   RefSeq; NP_178362.1; NM_126314.3.
DR   AlphaFoldDB; O64718; -.
DR   SMR; O64718; -.
DR   STRING; 3702.AT2G02580.1; -.
DR   PaxDb; O64718; -.
DR   PRIDE; O64718; -.
DR   ProteomicsDB; 239200; -.
DR   EnsemblPlants; AT2G02580.1; AT2G02580.1; AT2G02580.
DR   GeneID; 814788; -.
DR   Gramene; AT2G02580.1; AT2G02580.1; AT2G02580.
DR   KEGG; ath:AT2G02580; -.
DR   Araport; AT2G02580; -.
DR   TAIR; locus:2065254; AT2G02580.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_1_1; -.
DR   InParanoid; O64718; -.
DR   OMA; PLERMHI; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; O64718; -.
DR   BioCyc; ARA:AT2G02580-MON; -.
DR   PRO; PR:O64718; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O64718; baseline and differential.
DR   Genevisible; O64718; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..500
FT                   /note="Cytochrome P450 71B9"
FT                   /id="PRO_0000052087"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         440
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        321
FT                   /note="P -> A (in Ref. 3; AAL59946)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   500 AA;  57084 MW;  9D23C396410E8E4E CRC64;
     MATIWFLSLL FLCCILLAAF KHKKRRTNQQ QPPSPPGFPI IGNLHQLGEL PHQSLWSLSK
     TYGPVMLLKL GSVPTVVVSS SETAKQVLKI NDLHCCSRPS LAGAKELSYN YLDIAFSPFD
     DYWKELRRIC VQELFSAKRV HSIQPIKEEE VRKLIVSATE SASQKSPVNL SEKFLDLTVS
     VICKAAFSLD FHTSVLNNDG FDKLIHDAFL FLGSFSASNF FPNGGWIIDW LTGLQRRREK
     SVKDLDVFYQ QMFDLHKQEN KQGVEDFVDL LLKLEKEETV LGYGKLTRNH VKAILMNVLL
     GAINTSAMTM TWAMAELIRN PRVMKKVQSE IRNQMINKSV ITLDDIDHLP YLKMVIKETW
     RLHPPVPLLL PREVMSEFEI NGYKIQPKTL LYVNVWAIGR DPDSWKDADM FYPERFMDNN
     IDAKGQNFEL LPFGSGRRIC PGMYMGTTMV EFGLANMLYQ FDWEVPDGMV VEDIDMEESP
     GLAVGKKNEL LLVPVKYLGH