ID   C71BC_ARATH             Reviewed;         496 AA.
AC   Q9ZU07; A4VCM5;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Cytochrome P450 71B12;
DE            EC=1.14.-.-;
GN   Name=CYP71B12; OrderedLocusNames=At5g25130; ORFNames=F21J6.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista-Mercan V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C.,
RA   Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AC005964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC006259; AAC98444.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED93404.1; -; Genomic_DNA.
DR   EMBL; BT030466; ABP88120.1; -; mRNA.
DR   RefSeq; NP_197895.1; NM_122422.4.
DR   AlphaFoldDB; Q9ZU07; -.
DR   SMR; Q9ZU07; -.
DR   PaxDb; Q9ZU07; -.
DR   PRIDE; Q9ZU07; -.
DR   ProteomicsDB; 239135; -.
DR   EnsemblPlants; AT5G25130.1; AT5G25130.1; AT5G25130.
DR   GeneID; 832584; -.
DR   Gramene; AT5G25130.1; AT5G25130.1; AT5G25130.
DR   KEGG; ath:AT5G25130; -.
DR   Araport; AT5G25130; -.
DR   TAIR; locus:2179280; AT5G25130.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_1_1; -.
DR   InParanoid; Q9ZU07; -.
DR   OMA; IMAVACF; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; Q9ZU07; -.
DR   BioCyc; ARA:AT5G25130-MON; -.
DR   PRO; PR:Q9ZU07; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9ZU07; baseline and differential.
DR   Genevisible; Q9ZU07; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; HDA:TAIR.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..496
FT                   /note="Cytochrome P450 71B12"
FT                   /id="PRO_0000052090"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         436
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   496 AA;  56732 MW;  7361CBBF48ED617B CRC64;
     MSLWYIIVAF VFFSSMIIVR IIRKTKKNLP PGPPRLPIIG NLHQLGSKPH RSMFKLSETY
     GPLMSLKFGS VSTVVASTPE TVKEVLKTFD VECCSRPNMT YPARVTYNLK DLCFSPYSKY
     WREVRKMTVV ELYTAKRVQS FQHTRKEEVA ALVDFIKQAA SLEKPVNLNK KLMKLSGSVI
     CRVAFGINLQ GSKLENTYEE VIQGTVELVG SFAAADYFPV VGRIIDRITG LHSKCEKLFK
     AMDAFFDQSI KHHLEDEIIK DDIIDLLLKM ERGETTLGEF QLTRDHTKGI LANILNAGID
     TSAQVMTWVM TYLISNPRVL KKAQAEVREV IKHKDDIIEE DIERLQYLKM VIKETFRINP
     LVPLLIPREA SKDVKIGGYN IPKKTWIHVN IWAIHRNPNV WKDPEAFIPE RFMDSQIDYK
     GLNFELLPFG SGRRICPGIG MGMALVHLTL INLLYRFDWK LPEGMKVADV DLEESYGLVC
     PKKIPLQLIP VLTQWT