C71BV_ARATH
ID C71BV_ARATH Reviewed; 500 AA.
AC Q9LIP6; C0Z3G7;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Cytochrome P450 71B34;
DE EC=1.14.-.-;
GN Name=CYP71B34; OrderedLocusNames=At3g26300; ORFNames=F20C19.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LIP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LIP6-2; Sequence=VSP_037990, VSP_037991;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AP001298; BAB02190.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77143.1; -; Genomic_DNA.
DR EMBL; AY139766; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK319131; BAH57246.1; -; mRNA.
DR RefSeq; NP_189261.1; NM_113537.2. [Q9LIP6-1]
DR AlphaFoldDB; Q9LIP6; -.
DR SMR; Q9LIP6; -.
DR STRING; 3702.AT3G26300.1; -.
DR PaxDb; Q9LIP6; -.
DR PRIDE; Q9LIP6; -.
DR ProteomicsDB; 240303; -. [Q9LIP6-1]
DR EnsemblPlants; AT3G26300.1; AT3G26300.1; AT3G26300. [Q9LIP6-1]
DR GeneID; 822234; -.
DR Gramene; AT3G26300.1; AT3G26300.1; AT3G26300. [Q9LIP6-1]
DR KEGG; ath:AT3G26300; -.
DR Araport; AT3G26300; -.
DR TAIR; locus:2079251; AT3G26300.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; Q9LIP6; -.
DR OMA; TISLTRC; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9LIP6; -.
DR BioCyc; ARA:AT3G26300-MON; -.
DR PRO; PR:Q9LIP6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LIP6; baseline and differential.
DR Genevisible; Q9LIP6; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..500
FT /note="Cytochrome P450 71B34"
FT /id="PRO_0000052108"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 440
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VAR_SEQ 297..310
FT /note="DVLLAGMDTSAITM -> VTNHIFTIKSVRKI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_037990"
FT VAR_SEQ 311..500
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_037991"
FT CONFLICT 63
FT /note="G -> D (in Ref. 4; BAH57246)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="N -> S (in Ref. 4; BAH57246)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 57139 MW; 0A75380A20A53BA2 CRC64;
MTNIWLLSLI FVICILVAVF NHKNRRNYQR TPPSPPGCPI IGNLHQLGEL PHQSLWKLSK
KYGPVMLLKL GRVPTVIVSS SETAKQALKI HDLHCCSRPG FAGARELSYN YLDIAFSPYD
DYWKEVRKLA VQELFSSKQV HSIQPIKDEE VKKLIDSISE SAAQKTPINL NKTLLALTVS
VVCRTAFSVN FEGTVLNSER FNNIVREALE MLGSFSASDF IPYVGRIIDL LTGLQGRRER
SMRDLDAFYE QMFDLHKQKK EEGSEDFVDL LLRLEKEEAV LGNDKLTRNH IKAILMDVLL
AGMDTSAITM TWAMAELAKN PRVMKKVQSE IRSQIKNKER ISFDDTDKLE YLKMVIKETW
RLHPTTPLLI PREAMSEFEI NGYTIPVKTR LHVNVWAIGR DPDTWKDPEV FLPERFTDNN
IDAKGQHFEL LPFGGGRRMC PAVYMGTTMV EFGLANLLYH FDWKLPEGMK VDDIDMEEAP
GLTVNKKNEL ILVPTKFLDP
