ID   TYSY_METAC              Reviewed;         217 AA.
AC   Q8THH4;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   15-NOV-2002, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN   Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=MA_4543;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC       cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC       Rule:MF_01686}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC       ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM07882.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE010299; AAM07882.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_048066013.1; NC_003552.1.
DR   AlphaFoldDB; Q8THH4; -.
DR   SMR; Q8THH4; -.
DR   STRING; 188937.MA_4543; -.
DR   EnsemblBacteria; AAM07882; AAM07882; MA_4543.
DR   GeneID; 1476437; -.
DR   KEGG; mac:MA_4543; -.
DR   HOGENOM; CLU_084975_0_0_2; -.
DR   InParanoid; Q8THH4; -.
DR   OMA; HHSVSMH; -.
DR   OrthoDB; 57461at2157; -.
DR   PhylomeDB; Q8THH4; -.
DR   UniPathway; UPA00575; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004799; F:thymidylate synthase activity; IBA:GO_Central.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR   Gene3D; 3.30.572.10; -; 1.
DR   HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR   InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   InterPro; IPR014620; Thymidylate_synthase_arc.
DR   PANTHER; PTHR11548; PTHR11548; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PIRSF; PIRSF036752; TSase_MJ051; 1.
DR   SUPFAM; SSF55831; SSF55831; 1.
DR   TIGRFAMs; TIGR03283; thy_syn_methano; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..217
FT                   /note="Putative thymidylate synthase"
FT                   /id="PRO_0000141054"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ   SEQUENCE   217 AA;  25159 MW;  D2701C76252E52D5 CRC64;
     MEDKFEIGRI IRAKNISDAW YRGLNIIWNH GQVITDERGS QIREFMDLMV VIENPYTDRI
     PEDTAWNEER LEEYAKQLIS GENAQDFEYT YGQRLRNWNE EVDQIEYVIE KLQESPTSRR
     ATAVTWIPPV DTKVNEVPCM ILDDFKIRDG KVHLTTLFRS HDFGGAYPAN LYGLSKLLEY
     VAGRVGVEPG MITTVSISAH VYDHDWDMVE NIVKGIN