ID   TYSY_METBF              Reviewed;         217 AA.
AC   Q46E28;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN   Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=Mbar_A0890;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC       cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC       Rule:MF_01686}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC       ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
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DR   EMBL; CP000099; AAZ69864.1; -; Genomic_DNA.
DR   RefSeq; WP_011305913.1; NC_007355.1.
DR   AlphaFoldDB; Q46E28; -.
DR   SMR; Q46E28; -.
DR   STRING; 269797.Mbar_A0890; -.
DR   EnsemblBacteria; AAZ69864; AAZ69864; Mbar_A0890.
DR   GeneID; 3625935; -.
DR   KEGG; mba:Mbar_A0890; -.
DR   eggNOG; arCOG03214; Archaea.
DR   HOGENOM; CLU_084975_0_0_2; -.
DR   OMA; HHSVSMH; -.
DR   OrthoDB; 57461at2157; -.
DR   UniPathway; UPA00575; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR   Gene3D; 3.30.572.10; -; 1.
DR   HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR   InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   InterPro; IPR014620; Thymidylate_synthase_arc.
DR   PANTHER; PTHR11548; PTHR11548; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PIRSF; PIRSF036752; TSase_MJ051; 1.
DR   SUPFAM; SSF55831; SSF55831; 1.
DR   TIGRFAMs; TIGR03283; thy_syn_methano; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Transferase.
FT   CHAIN           1..217
FT                   /note="Putative thymidylate synthase"
FT                   /id="PRO_1000000625"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ   SEQUENCE   217 AA;  25233 MW;  5CC1C52E9FCA464B CRC64;
     MEDRFEIGRI VRAKTISDAW YRGLNIIRNH GQVITDERGS QIREFMDLMI IIEDPYTDRI
     PHETAWNEER LEEYAKQLIS GENTQDFEYT YGQRLRNWHG KVDQIDYVIE KLQQNPTSRR
     AIAVTWIPPV DTKVNEVPCM MLDDFKIRDG KIHLTTLFRS HDFGGAYPAN LYGLSKLLEY
     VADKVGTKPG TITTISISAH IYDHDWDMVE NIVKGVN