ID   TYSY_METJA              Reviewed;         222 AA.
AC   Q57931;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN   Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=MJ0511;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC       cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC       Rule:MF_01686}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC       ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
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DR   EMBL; L77117; AAB98502.1; -; Genomic_DNA.
DR   PIR; G64363; G64363.
DR   RefSeq; WP_010870012.1; NC_000909.1.
DR   AlphaFoldDB; Q57931; -.
DR   SMR; Q57931; -.
DR   STRING; 243232.MJ_0511; -.
DR   EnsemblBacteria; AAB98502; AAB98502; MJ_0511.
DR   GeneID; 1451373; -.
DR   KEGG; mja:MJ_0511; -.
DR   eggNOG; arCOG03214; Archaea.
DR   HOGENOM; CLU_084975_0_0_2; -.
DR   InParanoid; Q57931; -.
DR   OMA; HHSVSMH; -.
DR   OrthoDB; 57461at2157; -.
DR   PhylomeDB; Q57931; -.
DR   UniPathway; UPA00575; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004799; F:thymidylate synthase activity; IBA:GO_Central.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR   Gene3D; 3.30.572.10; -; 1.
DR   HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR   InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   InterPro; IPR014620; Thymidylate_synthase_arc.
DR   InterPro; IPR020940; Thymidylate_synthase_AS.
DR   PANTHER; PTHR11548; PTHR11548; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PIRSF; PIRSF036752; TSase_MJ051; 1.
DR   SUPFAM; SSF55831; SSF55831; 1.
DR   TIGRFAMs; TIGR03283; thy_syn_methano; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..222
FT                   /note="Putative thymidylate synthase"
FT                   /id="PRO_0000141055"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ   SEQUENCE   222 AA;  25933 MW;  3BC5E8C38237CE92 CRC64;
     MLCIKKPSVA SAFNELIPKI LKDGEVVETE FEERTKEIRN TIIEITNPKL KKVPEKYPLG
     EKAVEEYTKN LLYGSKNVFS YDYHQRLFEY PYADEKINQI DYIIEKLNQQ KNSRRAVAIT
     WNPKIDIEVS RDERGSVPCL QLVQFLIRNG KLYQTVIFRS NDALLAFVSN AIGLITLGEY
     IAKKVGVGYG TYTHHAISMH IYVDRDFDYI KKYFPECLKY LW