TYSY_METKA
ID TYSY_METKA Reviewed; 209 AA.
AC Q8TVL9;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=MK1370;
OS Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC Methanopyrus.
OX NCBI_TaxID=190192;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX PubMed=11930014; DOI=10.1073/pnas.032671499;
RA Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA Koonin E.V., Kozyavkin S.A.;
RT "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT monophyly of archaeal methanogens.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC Rule:MF_01686}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
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DR EMBL; AE009439; AAM02583.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8TVL9; -.
DR SMR; Q8TVL9; -.
DR STRING; 190192.MK1370; -.
DR EnsemblBacteria; AAM02583; AAM02583; MK1370.
DR KEGG; mka:MK1370; -.
DR HOGENOM; CLU_084975_0_0_2; -.
DR UniPathway; UPA00575; -.
DR Proteomes; UP000001826; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR Gene3D; 3.30.572.10; -; 1.
DR HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR InterPro; IPR014620; Thymidylate_synthase_arc.
DR PANTHER; PTHR11548; PTHR11548; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PIRSF; PIRSF036752; TSase_MJ051; 1.
DR SUPFAM; SSF55831; SSF55831; 1.
DR TIGRFAMs; TIGR03283; thy_syn_methano; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..209
FT /note="Putative thymidylate synthase"
FT /id="PRO_0000141056"
FT ACT_SITE 137
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ SEQUENCE 209 AA; 23781 MW; 08A0674BBBA8AB39 CRC64;
MLGKGRPLVV RGRTVEQVWR QAVTGIKVHG EKVERERGPV KEVRGLIAHL EPSGPESFDI
PDDYPLDEHS VRAYEDQLLD PELRGFEYTY GHRLRRYFGL DQVTKIVERL SESNNTRRAI
AVTWDPRRDL DEEEVPCLTA LQLQSDGGSG LELHAFYRSW DVGKALVANM IALRRLQEHV
AERAGLEPTT LTVYAANAHV YEEDLPDLP