TYSY_METMA
ID TYSY_METMA Reviewed; 217 AA.
AC Q8PXI1;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=MM_1237;
OS Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS 11833 / OCM 88) (Methanosarcina frisia).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=192952;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX PubMed=12125824;
RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA Fritz H.-J., Gottschalk G.;
RT "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT between Bacteria and Archaea.";
RL J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC Rule:MF_01686}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
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DR EMBL; AE008384; AAM30933.1; -; Genomic_DNA.
DR RefSeq; WP_011033186.1; NC_003901.1.
DR AlphaFoldDB; Q8PXI1; -.
DR SMR; Q8PXI1; -.
DR STRING; 192952.MM_1237; -.
DR PRIDE; Q8PXI1; -.
DR EnsemblBacteria; AAM30933; AAM30933; MM_1237.
DR GeneID; 24876213; -.
DR KEGG; mma:MM_1237; -.
DR PATRIC; fig|192952.21.peg.1442; -.
DR eggNOG; arCOG03214; Archaea.
DR HOGENOM; CLU_084975_0_0_2; -.
DR OMA; HHSVSMH; -.
DR UniPathway; UPA00575; -.
DR Proteomes; UP000000595; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR Gene3D; 3.30.572.10; -; 1.
DR HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR InterPro; IPR014620; Thymidylate_synthase_arc.
DR PANTHER; PTHR11548; PTHR11548; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PIRSF; PIRSF036752; TSase_MJ051; 1.
DR SUPFAM; SSF55831; SSF55831; 1.
DR TIGRFAMs; TIGR03283; thy_syn_methano; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..217
FT /note="Putative thymidylate synthase"
FT /id="PRO_0000141057"
FT ACT_SITE 139
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ SEQUENCE 217 AA; 25154 MW; AD36E8C724C119E6 CRC64;
MENKFEIGRI IRAKNISDAW YRGLNIIWNH GRVITDERGS QIKEFMDLMV VIENPYTDRI
PADTAWNEER LEEYAKQLIS GENIQDFEYT YGQRLRNWNG EVDQIEYVIE KLKESPTSRR
ATAVTWIPPV DTKVNEVPCM ILDDFKIRDE KVHLTTLFRS HDFGGAYPAN LYGLSKLLEY
VAEKVGVEPG VITTVSISAH VYDHDWDMVE NIVKGIN
