ID   TYSY_METTH              Reviewed;         222 AA.
AC   O26868;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Putative thymidylate synthase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TS {ECO:0000255|HAMAP-Rule:MF_01686};
DE            Short=TSase {ECO:0000255|HAMAP-Rule:MF_01686};
DE            EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_01686};
GN   Name=thyA {ECO:0000255|HAMAP-Rule:MF_01686}; OrderedLocusNames=MTH_774;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC       cosubstrate. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-
CC       Rule:MF_01686}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC       ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_01686}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB85277.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000666; AAB85277.1; ALT_INIT; Genomic_DNA.
DR   PIR; E69203; E69203.
DR   RefSeq; WP_048061245.1; NC_000916.1.
DR   AlphaFoldDB; O26868; -.
DR   SMR; O26868; -.
DR   STRING; 187420.MTH_774; -.
DR   EnsemblBacteria; AAB85277; AAB85277; MTH_774.
DR   GeneID; 1471182; -.
DR   KEGG; mth:MTH_774; -.
DR   PATRIC; fig|187420.15.peg.762; -.
DR   HOGENOM; CLU_084975_0_0_2; -.
DR   OMA; HHSVSMH; -.
DR   UniPathway; UPA00575; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR   Gene3D; 3.30.572.10; -; 1.
DR   HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR   InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   InterPro; IPR014620; Thymidylate_synthase_arc.
DR   PANTHER; PTHR11548; PTHR11548; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PIRSF; PIRSF036752; TSase_MJ051; 1.
DR   SUPFAM; SSF55831; SSF55831; 1.
DR   TIGRFAMs; TIGR03283; thy_syn_methano; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW   Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..222
FT                   /note="Putative thymidylate synthase"
FT                   /id="PRO_0000141058"
FT   ACT_SITE        146
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01686"
SQ   SEQUENCE   222 AA;  25556 MW;  1A01DE87215FC09B CRC64;
     MAYSIEVNEI ADGWKKLVEK IMHDGREIRD ERGSLTREVM NTVVTIKKPL GKSDDFYHIR
     RGSLLNIKVP EGYFWSGEKL EKYSEQFLSG DRKGFVYTYG NRLRAHFGVD QLDEAIRRLK
     NCTESRRATM VTWDPPEDTA SDEVPCMILV DFKIRDGRLF TTALWRSHDI YGAWFPNAVG
     LAYLADHVAA EVGVEVGHIT IHSISAHIYE VNFKEAEEVI KW