C71Z6_ORYSJ
ID C71Z6_ORYSJ Reviewed; 515 AA.
AC A3A871; Q6YV92;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Ent-isokaurene C2/C3-hydroxylase;
DE EC=1.14.14.76 {ECO:0000269|PubMed:21985968};
DE AltName: Full=Cytochrome P450 71Z6;
GN Name=CYP71Z6; OrderedLocusNames=Os02g0570500, LOC_Os02g36150;
GN ORFNames=OsJ_07206, OSJNBa0008E01.28;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-515.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18759039; DOI=10.1360/02yc9056;
RA Zhong L., Wang K., Tan J., Li W., Li S.;
RT "Putative cytochrome P450 genes in rice genome (Oryza sativa L. ssp.
RT indica) and their EST evidence.";
RL Sci. China, Ser. C, Life Sci. 45:512-517(2002).
RN [7]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=21985968; DOI=10.1016/j.febslet.2011.09.038;
RA Wu Y., Hillwig M.L., Wang Q., Peters R.J.;
RT "Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical
RT characterization of CYP71Z6 & 7.";
RL FEBS Lett. 585:3446-3451(2011).
CC -!- FUNCTION: Enzyme of the diterpenoid metabolism involved in the
CC biosynthesis of antibacterial oryzalides such as phytocassane.
CC {ECO:0000269|PubMed:21985968}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-isokaurene + 2 O2 + 2 reduced [NADPH--hemoprotein
CC reductase] = ent-isokaurene-2beta,3beta-diol + 2 H(+) + 2 H2O + 2
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:56336,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:50783,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:140275;
CC EC=1.14.14.76; Evidence={ECO:0000269|PubMed:21985968};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12 uM for ent-isokaurene {ECO:0000269|PubMed:21985968};
CC Note=kcat is 0.01 sec(-1) with ent-isokaurene as substrate.
CC {ECO:0000269|PubMed:21985968};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD17674.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAF09101.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Cytochrome P450 Homepage;
CC URL="http://drnelson.uthsc.edu/CytochromeP450.html";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP005835; BAD17674.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008208; BAF09101.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000139; EAZ23510.1; -; Genomic_DNA.
DR EMBL; AK107418; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015623817.1; XM_015768331.1.
DR AlphaFoldDB; A3A871; -.
DR SMR; A3A871; -.
DR STRING; 4530.OS02T0570500-01; -.
DR PaxDb; A3A871; -.
DR GeneID; 4329725; -.
DR KEGG; osa:4329725; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; A3A871; -.
DR OrthoDB; 1247045at2759; -.
DR BioCyc; MetaCyc:MON-18621; -.
DR BRENDA; 1.14.14.76; 8948.
DR PlantReactome; R-OSA-9610720; Oryzalide A biosynthesis.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; A3A871; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0036201; F:ent-isokaurene C2-hydroxylase activity; IDA:UniProtKB.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IDA:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IDA:UniProtKB.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..515
FT /note="Ent-isokaurene C2/C3-hydroxylase"
FT /id="PRO_0000418864"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 452
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 515 AA; 57157 MW; B2AC8C84F25C073A CRC64;
MEDKLILDLC LSALFVVVLS KLVSSAMKPR LNLPPGPWTL PLIGSLHHLV MTKSPQTHRS
LRALSEKHGP IMQLWMGEVP AVVVSSPAVA EEVLKHQDLR FADRHLTATT EEVFFGGRDV
IFGPYSERWR HLRKICMQEL LTAARVRSFQ GVREREVARL VRELAADAGA GGDAGVNLNE
RISKLANDIV MVSSVGGRCS HRDEFLDALE VAKKQITWLS VADLFPSSKL ARMVAVAPRK
GLASRKRMEL VIRRIIQERK DQLMDDSAAG AGEAAAGKDC FLDVLLRLQK EGGTPVPVTD
EIIVVLLFDM ISGASETSPT VLIWTLAELM RNPRIMAKAQ AEVRQAVAGK TTITEDDIVG
LSYLKMVIKE TLRLHPPAPL LNPRKCRETS QVMGYDIPKG TSVFVNMWAI CRDSRYWEDP
EEYKPERFEN NSVDYKGNNF EFLPFGSGRR ICPGINLGVA NLELPLASLL YHFDWKLPNG
MAPKDLDMHE TSGMVAAKLI TLNICPITHI APSSA
