TYW31_PYRHO
ID TYW31_PYRHO Reviewed; 196 AA.
AC O58796;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_00266};
DE EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 1 {ECO:0000255|HAMAP-Rule:MF_00266};
GN Name=taw3-1 {ECO:0000255|HAMAP-Rule:MF_00266}; OrderedLocusNames=PH1069;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS).
RA Lokanath N.K., Kunishima N.;
RT "Structure of PH1069 protein from Pyrococcus horikoshii.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC as a component of the wyosine derivatives biosynthesis pathway.
CC Probably methylates N-4 position of wybutosine-86 to produce
CC wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC Rule:MF_00266}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA30168.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000001; BAA30168.1; ALT_INIT; Genomic_DNA.
DR PIR; B71101; B71101.
DR PDB; 2DRV; X-ray; 1.60 A; A/B=1-196.
DR PDB; 2IT2; X-ray; 1.50 A; A/B=1-196.
DR PDB; 2IT3; X-ray; 2.10 A; A/B=1-196.
DR PDBsum; 2DRV; -.
DR PDBsum; 2IT2; -.
DR PDBsum; 2IT3; -.
DR AlphaFoldDB; O58796; -.
DR SMR; O58796; -.
DR STRING; 70601.3257485; -.
DR EnsemblBacteria; BAA30168; BAA30168; BAA30168.
DR KEGG; pho:PH1069; -.
DR eggNOG; arCOG04156; Archaea.
DR OMA; GKWHHYA; -.
DR EvolutionaryTrace; O58796; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0008175; F:tRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1960.10; -; 1.
DR HAMAP; MF_00266; TYW3_archaea; 1.
DR InterPro; IPR022908; Taw3.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF111278; SSF111278; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; S-adenosyl-L-methionine; Transferase;
KW tRNA processing.
FT CHAIN 1..196
FT /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT demethylwyosine(37)-N(4))-methyltransferase 1"
FT /id="PRO_0000157096"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 7..23
FT /evidence="ECO:0007829|PDB:2IT2"
FT TURN 29..31
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 32..40
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 53..59
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:2IT3"
FT STRAND 69..76
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 80..87
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 92..100
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 103..110
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 111..123
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 140..144
FT /evidence="ECO:0007829|PDB:2IT2"
FT STRAND 149..155
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 163..190
FT /evidence="ECO:0007829|PDB:2IT2"
FT HELIX 191..194
FT /evidence="ECO:0007829|PDB:2IT2"
SQ SEQUENCE 196 AA; 22657 MW; 278D881CB9E73E2D CRC64;
MRFTENFERA KKEALMSLEI ALRKGEVDED IIPLLKKINS IENYFTTSSC SGRISVMEMP
HFGDKVNAKW LGKWHREVSL YEVLEAIKKH RSGQLWFLVR SPILHVGAKT LEDAVKLVNL
AVSCGFKYSN IKSISNKKLI VEIRSTERMD VLLGENGEIF VGEEYLNKIV EIANDQMRRF
KEKLKRLESK INALNR
