ID   TYW32_PYRAB             Reviewed;         206 AA.
AC   Q9V1Y5; G8ZHS4;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2 {ECO:0000255|HAMAP-Rule:MF_00266};
DE            EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE   AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 2 {ECO:0000255|HAMAP-Rule:MF_00266};
GN   Name=taw3-2 {ECO:0000255|HAMAP-Rule:MF_00266};
GN   OrderedLocusNames=PYRAB02890; ORFNames=PAB2159;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC       as a component of the wyosine derivatives biosynthesis pathway.
CC       Probably methylates N-4 position of wybutosine-86 to produce
CC       wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00266}.
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DR   EMBL; AJ248283; CAB49213.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE69667.1; -; Genomic_DNA.
DR   PIR; F75220; F75220.
DR   RefSeq; WP_010867413.1; NC_000868.1.
DR   AlphaFoldDB; Q9V1Y5; -.
DR   SMR; Q9V1Y5; -.
DR   STRING; 272844.PAB2159; -.
DR   EnsemblBacteria; CAB49213; CAB49213; PAB2159.
DR   GeneID; 1495180; -.
DR   KEGG; pab:PAB2159; -.
DR   PATRIC; fig|272844.11.peg.311; -.
DR   eggNOG; arCOG04156; Archaea.
DR   HOGENOM; CLU_047426_2_0_2; -.
DR   OMA; GFKYTTF; -.
DR   OrthoDB; 118297at2157; -.
DR   PhylomeDB; Q9V1Y5; -.
DR   BioCyc; MetaCyc:MON-19458; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   HAMAP; MF_00266; TYW3_archaea; 1.
DR   InterPro; IPR022908; Taw3.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN           1..206
FT                   /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT                   demethylwyosine(37)-N(4))-methyltransferase 2"
FT                   /id="PRO_0000157098"
SQ   SEQUENCE   206 AA;  23292 MW;  5A4986C0564DDE35 CRC64;
     MKAKREALIS LFTAIKEGKV DEDIIDLLML INSIKGVYTT SSCSGRIGII EEPSLGAKPL
     SRWLIKVHRP MEFEEAIDAL KKANKGIIFL KSQPPILHVV AENLEMAKLL HQIGLSSGFK
     YTTFKVISNR YLVEINGTEY LTVPLGRDGK VLVSEEYLKF AVEIGNEMLR RGKSRLPRLY
     KNFQELKEKV GEDELFIALK REILGT