ID   TYW32_PYRFU             Reviewed;         206 AA.
AC   Q8TZG0;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2 {ECO:0000255|HAMAP-Rule:MF_00266};
DE            EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE   AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 2 {ECO:0000255|HAMAP-Rule:MF_00266};
GN   Name=taw3-2 {ECO:0000255|HAMAP-Rule:MF_00266}; OrderedLocusNames=PF2034;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC       as a component of the wyosine derivatives biosynthesis pathway.
CC       Probably methylates N-4 position of wybutosine-86 to produce
CC       wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00266}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL82158.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE009950; AAL82158.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_014835510.1; NZ_CP023154.1.
DR   AlphaFoldDB; Q8TZG0; -.
DR   SMR; Q8TZG0; -.
DR   STRING; 186497.PF2034; -.
DR   EnsemblBacteria; AAL82158; AAL82158; PF2034.
DR   GeneID; 41713856; -.
DR   KEGG; pfu:PF2034; -.
DR   PATRIC; fig|186497.12.peg.2111; -.
DR   eggNOG; arCOG04156; Archaea.
DR   HOGENOM; CLU_047426_2_0_2; -.
DR   OMA; GFKYTTF; -.
DR   OrthoDB; 118297at2157; -.
DR   PhylomeDB; Q8TZG0; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   HAMAP; MF_00266; TYW3_archaea; 1.
DR   InterPro; IPR022908; Taw3.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..206
FT                   /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT                   demethylwyosine(37)-N(4))-methyltransferase 2"
FT                   /id="PRO_0000157099"
SQ   SEQUENCE   206 AA;  23469 MW;  33B0FBF85FE4D502 CRC64;
     MKAKREALLS LFQAIKENKV DEDIVELLLL INSIKGVYTT SSCSGRIGII EEPSLGAKPL
     SRWLIKVHRP IKFEEAKKAL KNAQKGLIFL KSQPPIFHVV TESLELARKI HEIGLSSGFK
     YTTYKVISRR YLVEINGTEY LTVPLGKDGK VFVTDEYLEF VIEIGNQMLM RGKSRLPRLR
     EKFEELKEEV GEDPLFTTLS TEKLSL