C724B_ORYSI
ID C724B_ORYSI Reviewed; 480 AA.
AC B8AV52;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Cytochrome P450 724B1 {ECO:0000250|UniProtKB:Q6F4F5};
DE Short=OsCYP724B1 {ECO:0000250|UniProtKB:Q6F4F5};
DE AltName: Full=(22S)-22-hydroxycampesterol synthase {ECO:0000250|UniProtKB:Q6F4F5};
DE EC=1.14.14.- {ECO:0000250|UniProtKB:Q6F4F5};
GN Name=CYP724B1 {ECO:0000250|UniProtKB:Q6F4F5};
GN ORFNames=OsI_16255 {ECO:0000312|EMBL:EEC77448.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Involved in brassinosteroid biosynthesis (By similarity).
CC Involved in internode elongation and seed development (By similarity).
CC Catalyzes the conversion of campesterol (CR) to (22S)-22-
CC hydroxycampesterol (22-OHCR, 22-hydroxyCR) (By similarity).
CC {ECO:0000250|UniProtKB:Q6F4F5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=campesterol + O2 + reduced [NADPH--hemoprotein reductase] =
CC (22S)-22-hydroxycampesterol + H(+) + H2O + oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:69835, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:28623, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:72331;
CC Evidence={ECO:0000250|UniProtKB:Q6F4F5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69836;
CC Evidence={ECO:0000250|UniProtKB:Q6F4F5};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000250|UniProtKB:Q6F4F5}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; CM000129; EEC77448.1; -; Genomic_DNA.
DR SMR; B8AV52; -.
DR STRING; 39946.B8AV52; -.
DR EnsemblPlants; BGIOSGA014915-TA; BGIOSGA014915-PA; BGIOSGA014915.
DR Gramene; BGIOSGA014915-TA; BGIOSGA014915-PA; BGIOSGA014915.
DR HOGENOM; CLU_001570_15_5_1; -.
DR OMA; PWRWETQ; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000007015; Chromosome 4.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008199; F:ferric iron binding; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IEA:InterPro.
DR GO; GO:0006826; P:iron ion transport; IEA:InterPro.
DR GO; GO:0009647; P:skotomorphogenesis; IEA:EnsemblPlants.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR002024; Bacterioferritin.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00549; BACTERIOFERRITIN; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..480
FT /note="Cytochrome P450 724B1"
FT /id="PRO_0000455305"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 426
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 480 AA; 53704 MW; AD7FA92538822CC3 CRC64;
MVGGELVLAA LVILLALLLT LVLSHFLPLL LNPKAPKGSF GWPLLGETLR FLSPHASNTL
GSFLEDHCSR YGRVFKSHLF CTPTIVSCDQ ELNHFILQNE ERLFQCSYPR PIHGILGKSS
MLVVLGEDHK RLRNLALALV TSTKLKPSYL GDIEKIALHI VGSWHGKSKD KGMVNVIAFC
EEARKFAFSV IVKQVLGLSP EEPVTAMILE DFLAFMKGLI SFPLYIPGTP YAKAVQARAR
ISSTVKGIIE ERRNAGSSNK GDFLDVLLSS NELSDEEKVS FVLDSLLGGY ETTSLLISMV
VYFLGQSAQD LELVKREHEG IRSKKEKDEF LSSEDYKKME YTQHVINEAL RCGNIVKFVH
RKALKDVRYK EYLIPSGWKV LPVFSAVHLN PLLHGNAQQF QPCRWEGASQ GTSKKFTPFG
GGPRLCPGSE LAKVEAAFFL HHLVLNYRWR IDGDDIPMAY PYVEFQRGLP IEIEPLCSES
