TYW3_ARCFU
ID TYW3_ARCFU Reviewed; 196 AA.
AC O28220;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00266};
DE EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 {ECO:0000255|HAMAP-Rule:MF_00266};
GN Name=taw3 {ECO:0000255|HAMAP-Rule:MF_00266}; OrderedLocusNames=AF_2059;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RG Joint Center for Structural Genomics (JCSG);
RT "Crystal structure of uncharacterized protein AF2059 (2648472) from
RT Archaeoglobus fulgidus at 1.95 A resolution.";
RL Submitted (JUN-2007) to the PDB data bank.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC as a component of the wyosine derivatives biosynthesis pathway.
CC Probably methylates N-4 position of wybutosine-86 to produce
CC wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC Rule:MF_00266}.
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DR EMBL; AE000782; AAB89192.1; -; Genomic_DNA.
DR PIR; B69507; B69507.
DR RefSeq; WP_010879551.1; NC_000917.1.
DR PDB; 2QG3; X-ray; 1.95 A; A/B=1-196.
DR PDBsum; 2QG3; -.
DR AlphaFoldDB; O28220; -.
DR SMR; O28220; -.
DR STRING; 224325.AF_2059; -.
DR EnsemblBacteria; AAB89192; AAB89192; AF_2059.
DR GeneID; 24795808; -.
DR KEGG; afu:AF_2059; -.
DR eggNOG; arCOG04156; Archaea.
DR HOGENOM; CLU_047426_2_0_2; -.
DR OMA; GKWHHYA; -.
DR OrthoDB; 118297at2157; -.
DR PhylomeDB; O28220; -.
DR EvolutionaryTrace; O28220; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0008175; F:tRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1960.10; -; 1.
DR HAMAP; MF_00266; TYW3_archaea; 1.
DR InterPro; IPR022908; Taw3.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF111278; SSF111278; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase; tRNA processing.
FT CHAIN 1..196
FT /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT demethylwyosine(37)-N(4))-methyltransferase"
FT /id="PRO_0000157092"
FT HELIX 3..19
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 28..35
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 49..57
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 65..72
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 76..83
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 87..95
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 98..105
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 106..119
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:2QG3"
FT TURN 129..132
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 133..136
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 142..148
FT /evidence="ECO:0007829|PDB:2QG3"
FT STRAND 151..154
FT /evidence="ECO:0007829|PDB:2QG3"
FT HELIX 156..188
FT /evidence="ECO:0007829|PDB:2QG3"
FT TURN 189..193
FT /evidence="ECO:0007829|PDB:2QG3"
SQ SEQUENCE 196 AA; 22080 MW; 6FAC32B27E408671 CRC64;
MMWEQFKKEK LRGYLEAKNQ RKVDFDIVEL LDLINSFDDF VTLSSCSGRI AVVDLEKPGD
KASSLFLGKW HEGVEVSEVA EAALRSRKVA WLIQYPPIIH VACRNIGAAK LLMNAANTAG
FRRSGVISLS NYVVEIASLE RIELPVAEKG LMLVDDAYLS YVVRWANEKL LKGKEKLGRL
QEALESLQRE NAYCSD
