ID   TYW3_PIG                Reviewed;         259 AA.
AC   Q19QT9;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=tRNA wybutosine-synthesizing protein 3 homolog;
DE            Short=tRNA-yW-synthesizing protein 3;
DE            EC=2.1.1.282;
DE   AltName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase;
GN   Name=TYW3;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=16978188; DOI=10.1111/j.1365-2052.2006.01499.x;
RA   Kim J.H., Lim H.T., Park E.W., Ovilo C., Lee J.H., Jeon J.T.;
RT   "A gene-based radiation hybrid map of the pig chromosome 6q32 region
RT   associated with a QTL for fat deposition traits.";
RL   Anim. Genet. 37:522-523(2006).
CC   -!- FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase
CC       that acts as a component of the wybutosine biosynthesis pathway.
CC       Wybutosine is a hyper modified guanosine with a tricyclic base found at
CC       the 3'-position adjacent to the anticodon of eukaryotic phenylalanine
CC       tRNA (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC   -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000305}.
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DR   EMBL; DQ499447; ABF72037.1; -; mRNA.
DR   RefSeq; NP_001038048.1; NM_001044583.1.
DR   AlphaFoldDB; Q19QT9; -.
DR   SMR; Q19QT9; -.
DR   Ensembl; ENSSSCT00000039592; ENSSSCP00000042568; ENSSSCG00000003779.
DR   Ensembl; ENSSSCT00005059292; ENSSSCP00005036606; ENSSSCG00005036973.
DR   Ensembl; ENSSSCT00015014797; ENSSSCP00015005745; ENSSSCG00015011192.
DR   Ensembl; ENSSSCT00025012435; ENSSSCP00025004924; ENSSSCG00025009383.
DR   Ensembl; ENSSSCT00035022635; ENSSSCP00035008326; ENSSSCG00035017593.
DR   Ensembl; ENSSSCT00045057900; ENSSSCP00045040472; ENSSSCG00045033765.
DR   Ensembl; ENSSSCT00050088610; ENSSSCP00050038002; ENSSSCG00050065054.
DR   Ensembl; ENSSSCT00055028534; ENSSSCP00055022737; ENSSSCG00055014420.
DR   Ensembl; ENSSSCT00070033331; ENSSSCP00070027842; ENSSSCG00070016920.
DR   GeneID; 733652; -.
DR   KEGG; ssc:733652; -.
DR   CTD; 127253; -.
DR   VGNC; VGNC:94622; TYW3.
DR   GeneTree; ENSGT00940000153304; -.
DR   InParanoid; Q19QT9; -.
DR   OMA; GKWHHYA; -.
DR   OrthoDB; 1313208at2759; -.
DR   UniPathway; UPA00375; -.
DR   Proteomes; UP000008227; Chromosome 6.
DR   Proteomes; UP000314985; Chromosome 6.
DR   Bgee; ENSSSCG00000003779; Expressed in semimembranosus muscle and 46 other tissues.
DR   ExpressionAtlas; Q19QT9; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..259
FT                   /note="tRNA wybutosine-synthesizing protein 3 homolog"
FT                   /id="PRO_0000281844"
FT   REGION          200..259
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        202..237
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   259 AA;  29480 MW;  C547773892B31BF4 CRC64;
     MDRRAEFKRW KAQCLSKADF SRKGCVDEDV VEIVQLLNGR EQFFTTSSCA GRIILLEQGM
     DSLEVQKQNC CWLLVSHKPC VKDDVIVALK KANGDAILKF EPLVLHVQCR QLQDAQILHS
     VAIDSGFRNS GITVGKRGKT MLAVRSTHGL EVPLSHKGKL MVTEEYIDFL LKIANHKMEE
     NKKRIERFYN CLQLALEKET TTTNSHPKEK IKEKNNSYKK KSNPGKAHDK CVTEENDKVL
     ENDDDDDPGI SVTIFPEDY