ID   TYW3_PONAB              Reviewed;         258 AA.
AC   Q5R5S9;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=tRNA wybutosine-synthesizing protein 3 homolog;
DE            Short=tRNA-yW-synthesizing protein 3;
DE            EC=2.1.1.282;
DE   AltName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase;
GN   Name=TYW3;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase
CC       that acts as a component of the wybutosine biosynthesis pathway.
CC       Wybutosine is a hyper modified guanosine with a tricyclic base found at
CC       the 3'-position adjacent to the anticodon of eukaryotic phenylalanine
CC       tRNA (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC   -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000305}.
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DR   EMBL; CR860775; CAH92887.1; -; mRNA.
DR   RefSeq; NP_001126692.1; NM_001133220.1.
DR   RefSeq; XP_009247177.1; XM_009248902.1.
DR   RefSeq; XP_009247178.1; XM_009248903.1.
DR   AlphaFoldDB; Q5R5S9; -.
DR   SMR; Q5R5S9; -.
DR   STRING; 9601.ENSPPYP00000001435; -.
DR   Ensembl; ENSPPYT00000001480; ENSPPYP00000001435; ENSPPYG00000001234.
DR   GeneID; 100173692; -.
DR   KEGG; pon:100173692; -.
DR   CTD; 127253; -.
DR   eggNOG; KOG1228; Eukaryota.
DR   GeneTree; ENSGT00940000153304; -.
DR   HOGENOM; CLU_047426_1_1_1; -.
DR   InParanoid; Q5R5S9; -.
DR   OrthoDB; 1313208at2759; -.
DR   TreeFam; TF329327; -.
DR   UniPathway; UPA00375; -.
DR   Proteomes; UP000001595; Chromosome 1.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; Phosphoprotein; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN           1..258
FT                   /note="tRNA wybutosine-synthesizing protein 3 homolog"
FT                   /id="PRO_0000281845"
FT   MOD_RES         25
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8BSA9"
SQ   SEQUENCE   258 AA;  29422 MW;  7B35243CAA68633C CRC64;
     MDRSAEFRKW KAQCLSKADL SRKGSVDEDV VELVQFLNMR DQFFTTSSCA GRILLLDGGI
     NGFEVQKQNC CWLLVTHKLC VKDDVIVALK KANGDATLKF EPFVLHVQCR QLQDAQILHS
     VAIDAGFRNS GITVGKRGKT MLAVRSTHGL EVPLSHKGKL MVTEEYIDFL LNVANQKMEE
     NKKRIERFYN CLQHALERET MTNLHPKIKE KNNSSYTHKK KNPEKACAQC ITKENDKELE
     NDDDDDLGIN VTIFPEDY