TYW3_SACS2
ID TYW3_SACS2 Reviewed; 209 AA.
AC Q9UX16;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00266};
DE EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 {ECO:0000255|HAMAP-Rule:MF_00266};
GN Name=taw3 {ECO:0000255|HAMAP-Rule:MF_00266}; OrderedLocusNames=SSO0622;
GN ORFNames=C08_028;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=10701121; DOI=10.1139/g99-108;
RA Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C.,
RA Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T.,
RA Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E.,
RA Medina N., Peng X., Penny S.L., She Q., St Jean A., van der Oost J.,
RA Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.;
RT "Gene content and organization of a 281-kbp contig from the genome of the
RT extremely thermophilic archaeon, Sulfolobus solfataricus P2.";
RL Genome 43:116-136(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS).
RA Jia Z., Wong A.H.Y.;
RT "Crystal structure of conserved hypothetical protein SSO0622 from
RT Sulfolobus solfataricus.";
RL Submitted (JUN-2004) to the PDB data bank.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC as a component of the wyosine derivatives biosynthesis pathway.
CC Probably methylates N-4 position of wybutosine-86 to produce
CC wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC Rule:MF_00266}.
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DR EMBL; Y18930; CAB57678.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK40933.1; -; Genomic_DNA.
DR PIR; F90209; F90209.
DR RefSeq; WP_009991156.1; NC_002754.1.
DR PDB; 1TLJ; X-ray; 2.80 A; A/B=1-209.
DR PDBsum; 1TLJ; -.
DR AlphaFoldDB; Q9UX16; -.
DR SMR; Q9UX16; -.
DR STRING; 273057.SSO0622; -.
DR EnsemblBacteria; AAK40933; AAK40933; SSO0622.
DR GeneID; 44129624; -.
DR KEGG; sso:SSO0622; -.
DR eggNOG; arCOG04156; Archaea.
DR HOGENOM; CLU_047426_2_1_2; -.
DR InParanoid; Q9UX16; -.
DR OMA; GKWHHYA; -.
DR PhylomeDB; Q9UX16; -.
DR EvolutionaryTrace; Q9UX16; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008175; F:tRNA methyltransferase activity; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1960.10; -; 1.
DR HAMAP; MF_00266; TYW3_archaea; 1.
DR InterPro; IPR022908; Taw3.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF111278; SSF111278; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase; tRNA processing.
FT CHAIN 1..209
FT /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT demethylwyosine(37)-N(4))-methyltransferase"
FT /id="PRO_0000157102"
FT HELIX 3..20
FT /evidence="ECO:0007829|PDB:1TLJ"
FT HELIX 26..28
FT /evidence="ECO:0007829|PDB:1TLJ"
FT HELIX 29..37
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 40..48
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 51..59
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 67..74
FT /evidence="ECO:0007829|PDB:1TLJ"
FT HELIX 78..86
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 90..98
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 101..108
FT /evidence="ECO:0007829|PDB:1TLJ"
FT HELIX 109..122
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 128..133
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 136..141
FT /evidence="ECO:0007829|PDB:1TLJ"
FT STRAND 146..154
FT /evidence="ECO:0007829|PDB:1TLJ"
FT HELIX 161..189
FT /evidence="ECO:0007829|PDB:1TLJ"
SQ SEQUENCE 209 AA; 24115 MW; EA94C8B46E1FB88B CRC64;
MLVWEELREK ALNKIYHDKE IGYLDPDILG FLLAFYRNRN DVYTQSSCSG RITIVDAEMP
WDRKNSTIIF KNHLRITEQD LEDVLSKNQV RRLWLIVQGP IIHIYAKNIE TGWDILKIAR
EAGFKHSGIL ATNQKGVLVE LRTGIRMVHL LRESNTERVD KDKIKTLVNV CNEVLARGKQ
KMNLLKDLLS SSSNNSMELG KNSKLKTPI
