ID   TYW3_THESM              Reviewed;         197 AA.
AC   C6A5A3;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00266};
DE            EC=2.1.1.282 {ECO:0000255|HAMAP-Rule:MF_00266};
DE   AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw3 {ECO:0000255|HAMAP-Rule:MF_00266};
GN   Name=taw3 {ECO:0000255|HAMAP-Rule:MF_00266}; OrderedLocusNames=TSIB_1747;
OS   Thermococcus sibiricus (strain DSM 12597 / MM 739).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=604354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12597 / MM 739;
RX   PubMed=19447963; DOI=10.1128/aem.00718-09;
RA   Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V.,
RA   Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.;
RT   "Metabolic versatility and indigenous origin of the archaeon Thermococcus
RT   sibiricus, isolated from a siberian oil reservoir, as revealed by genome
RT   analysis.";
RL   Appl. Environ. Microbiol. 75:4580-4588(2009).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that acts
CC       as a component of the wyosine derivatives biosynthesis pathway.
CC       Probably methylates N-4 position of wybutosine-86 to produce
CC       wybutosine-72. {ECO:0000255|HAMAP-Rule:MF_00266}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00266};
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00266}.
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DR   EMBL; CP001463; ACS90798.1; -; Genomic_DNA.
DR   RefSeq; WP_015850014.1; NC_012883.1.
DR   AlphaFoldDB; C6A5A3; -.
DR   SMR; C6A5A3; -.
DR   STRING; 604354.TSIB_1747; -.
DR   EnsemblBacteria; ACS90798; ACS90798; TSIB_1747.
DR   GeneID; 8096757; -.
DR   KEGG; tsi:TSIB_1747; -.
DR   eggNOG; arCOG04156; Archaea.
DR   HOGENOM; CLU_047426_2_0_2; -.
DR   OMA; GKWHHYA; -.
DR   OrthoDB; 118297at2157; -.
DR   Proteomes; UP000009079; Chromosome.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0031591; P:wybutosine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   HAMAP; MF_00266; TYW3_archaea; 1.
DR   InterPro; IPR022908; Taw3.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..197
FT                   /note="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-
FT                   demethylwyosine(37)-N(4))-methyltransferase"
FT                   /id="PRO_1000204690"
SQ   SEQUENCE   197 AA;  22596 MW;  3C744C49262092B0 CRC64;
     MFLYEKNFDL QKKKALESLN DALEKGLVDS DIISLLNKIN SLKNYFTTSS CSGRISIMQM
     PDLGDKLNAI WLGKWHREVK IEEVLDTINK HDGGMLWFMV HSPILHVSAK TLEDAVELLN
     LAMACGFKHS NIKSVSHKKL VVEIRSTERM DIPLGSDGEL WVNESYLVKI VSMANLQLRR
     TKEKLRKLED EIQKLKK