ID   TYW3_XENLA              Reviewed;         251 AA.
AC   Q641F8;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=tRNA wybutosine-synthesizing protein 3 homolog;
DE            Short=tRNA-yW-synthesizing protein 3;
DE            EC=2.1.1.282;
DE   AltName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase;
GN   Name=tyw3;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase
CC       that acts as a component of the wybutosine biosynthesis pathway.
CC       Wybutosine is a hyper modified guanosine with a tricyclic base found at
CC       the 3'-position adjacent to the anticodon of eukaryotic phenylalanine
CC       tRNA (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC         tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC         carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC         COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC   -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC   -!- SIMILARITY: Belongs to the TYW3 family. {ECO:0000305}.
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DR   EMBL; BC082379; AAH82379.1; -; mRNA.
DR   RefSeq; NP_001087853.1; NM_001094384.1.
DR   AlphaFoldDB; Q641F8; -.
DR   SMR; Q641F8; -.
DR   DNASU; 447714; -.
DR   GeneID; 447714; -.
DR   KEGG; xla:447714; -.
DR   CTD; 447714; -.
DR   Xenbase; XB-GENE-5729656; tyw3.L.
DR   OrthoDB; 1313208at2759; -.
DR   UniPathway; UPA00375; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 447714; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1960.10; -; 1.
DR   InterPro; IPR003827; tRNA_yW-synthesising.
DR   InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR   Pfam; PF02676; TYW3; 1.
DR   SUPFAM; SSF111278; SSF111278; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..251
FT                   /note="tRNA wybutosine-synthesizing protein 3 homolog"
FT                   /id="PRO_0000281847"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          209..251
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        228..245
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   251 AA;  28693 MW;  DFF9D68122C720E3 CRC64;
     MEKQEAFSHW KQQSARKTDV SKKGSVDEDI EETVRLINQQ ERYFTTSSCS GRVIIINETL
     DNSTIQKQNC SWLFVTHKLC KPDDVFAALQ NATGDVVLKF EPFVLHVQCR ALEDAQLLHS
     VAINAGFRNS GITVGKKGKI IMAVRSTHGL EVPLTQNGKC LVSHEYIEFL VHTANKKMEE
     NKRRITRFHS CLHKALQKED CVHDKTSKEQ ASSCVYVRRR KRKDRSRKSN CSSSEEEHTD
     SEPELTLFND P