U1148_ARATH
ID U1148_ARATH Reviewed; 712 AA.
AC Q9M8X2; F4J3L0;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=U11/U12 small nuclear ribonucleoprotein 48 kDa protein;
DE Short=U11/U12 snRNP 48 kDa protein;
DE Short=U11/U12-48K;
GN Name=SNRNP48; OrderedLocusNames=At3g04160; ORFNames=T6K12.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION.
RX PubMed=15987817; DOI=10.1261/rna.2440305;
RA Lorkovic Z.J., Lehner R., Forstner C., Barta A.;
RT "Evolutionary conservation of minor U12-type spliceosome between plants and
RT humans.";
RL RNA 11:1095-1107(2005).
CC -!- FUNCTION: Likely involved in U12-type 5' splice site recognition.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome. Not found in the major spliceosome (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9M8X2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9M8X2-2; Sequence=VSP_055299;
CC -!- DOMAIN: The CHHC region interacts with the 5' splice site of the U12-
CC type intron. {ECO:0000250}.
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DR EMBL; AC016829; AAF26797.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74045.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74046.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64538.1; -; Genomic_DNA.
DR RefSeq; NP_001189804.1; NM_001202875.1. [Q9M8X2-2]
DR RefSeq; NP_001326557.1; NM_001337505.1. [Q9M8X2-1]
DR RefSeq; NP_187066.1; NM_111287.3. [Q9M8X2-1]
DR AlphaFoldDB; Q9M8X2; -.
DR STRING; 3702.AT3G04160.2; -.
DR iPTMnet; Q9M8X2; -.
DR PaxDb; Q9M8X2; -.
DR PRIDE; Q9M8X2; -.
DR ProteomicsDB; 242605; -. [Q9M8X2-1]
DR EnsemblPlants; AT3G04160.1; AT3G04160.1; AT3G04160. [Q9M8X2-1]
DR EnsemblPlants; AT3G04160.2; AT3G04160.2; AT3G04160. [Q9M8X2-2]
DR EnsemblPlants; AT3G04160.3; AT3G04160.3; AT3G04160. [Q9M8X2-1]
DR GeneID; 819571; -.
DR Gramene; AT3G04160.1; AT3G04160.1; AT3G04160. [Q9M8X2-1]
DR Gramene; AT3G04160.2; AT3G04160.2; AT3G04160. [Q9M8X2-2]
DR Gramene; AT3G04160.3; AT3G04160.3; AT3G04160. [Q9M8X2-1]
DR KEGG; ath:AT3G04160; -.
DR Araport; AT3G04160; -.
DR TAIR; locus:2103030; AT3G04160.
DR eggNOG; ENOG502QSM9; Eukaryota.
DR HOGENOM; CLU_367008_0_0_1; -.
DR OMA; MEHTYIS; -.
DR OrthoDB; 1626841at2759; -.
DR PhylomeDB; Q9M8X2; -.
DR PRO; PR:Q9M8X2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M8X2; baseline and differential.
DR Genevisible; Q9M8X2; AT.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR032845; Snrnp48.
DR InterPro; IPR022776; TRM13/UPF0224_CHHC_Znf_dom.
DR PANTHER; PTHR21402:SF10; PTHR21402:SF10; 1.
DR Pfam; PF05253; zf-U11-48K; 1.
DR PROSITE; PS51800; ZF_CHHC_U11_48K; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Metal-binding; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Ribonucleoprotein; Spliceosome; Zinc;
KW Zinc-finger.
FT CHAIN 1..712
FT /note="U11/U12 small nuclear ribonucleoprotein 48 kDa
FT protein"
FT /id="PRO_0000429829"
FT ZN_FING 98..125
FT /note="CHHC U11-48K-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01141"
FT REGION 562..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..586
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..665
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 675..693
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01141"
FT BINDING 107
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01141"
FT BINDING 117
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01141"
FT BINDING 121
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01141"
FT VAR_SEQ 460..472
FT /note="AAELGFMTAKADE -> IISCLHLSLIPHDVS (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_055299"
SQ SEQUENCE 712 AA; 81967 MW; 1C5488FB93C46E3B CRC64;
MDRPPSLPHY QNPNPNLFYH YPPPNSNPNF FFRPPPPPLQ NPNNYSIVPS PPPIRELSGT
LSSLKSLLSE CQRTLDSLSQ NLALDHSSLL QKDENGCFVR CPFDSNHFMP PEALFLHSLR
CPNTLDLIHL LESFSSYRNT LELPCELQLN NGDGDLCISL DDLADFGSNF FYRDCPGAVK
FSELDGKKRT LTLPHVLSVE CSDFVGSDEK VKKIVLDKCL GVLPSDLCAM KNEIDQWRDF
PSSYSSSVLS SIVGSKVVEI SALRKWILVN STRYGVIIDT FMRDHIFLLF RLCLKSAVKE
ACGFRMESDA TDVGEQKIMS CKSSTFECPV FIQVLSWLAS QLAVLYGEGN GKFFALDMFK
QCIVESASQV MLFRLEGTRS KCSGVVEDLD DARLRNKDVI MEKPFENSSG GECGKTLDSP
QVISVSRVSA AVAALYERSL LEEKIRAVRY AQPLTRYQRA AELGFMTAKA DEERNRRCSY
RPIIDHDGRP RQRSLNQDMD KMKTREELLA EERDYKRRRM SYRGKKVKRT PRQVLHDMIE
EYTEEIKLAG GIGCFEKGMP LQSRSPIGND QKESDFGYSI PSTDKQWKGE NRADIEYPID
NRQNSDKVKR HDEYDSGSSQ RQQSHRSYKH SDRRDDKLRD RRKDKHNDRR DDEFTRTKRH
SIEGESYQNY RSSREKSSSD YKTKRDDPYD RRSQQPRNQN LFEDRYIPTE KE
