U1165_ARATH
ID U1165_ARATH Reviewed; 442 AA.
AC Q8RWV8; C0Z2T6; O80484;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=U11/U12 small nuclear ribonucleoprotein 65 kDa protein;
DE Short=U11/U12 snRNP 65 kDa protein;
DE Short=U11/U12-65K;
GN Name=SNRNP65; OrderedLocusNames=At1g09230; ORFNames=T12M4.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP IDENTIFICATION.
RX PubMed=15987817; DOI=10.1261/rna.2440305;
RA Lorkovic Z.J., Lehner R., Forstner C., Barta A.;
RT "Evolutionary conservation of minor U12-type spliceosome between plants and
RT humans.";
RL RNA 11:1095-1107(2005).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE,
RP AND RNA-BINDING.
RC STRAIN=cv. Columbia;
RX PubMed=24606192; DOI=10.1111/tpj.12498;
RA Jung H.J., Kang H.;
RT "The Arabidopsis U11/U12-65K is an indispensible component of minor
RT spliceosome and plays a crucial role in U12 intron splicing and plant
RT development.";
RL Plant J. 78:799-810(2014).
CC -!- FUNCTION: Component of minor spliceosome required for U12-type intron
CC splicing and alternative splicing of many introns. Binds specifically
CC to U12 snRNA, which is necessary for branch-point site recognition.
CC Required for normal plant development. {ECO:0000269|PubMed:24606192}.
CC -!- SUBUNIT: Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome. Forms a complex with U12 snRNA.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24606192}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RWV8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RWV8-2; Sequence=VSP_055303, VSP_055304;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:24606192}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous.
CC {ECO:0000269|PubMed:24606192}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24093.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003114; AAC24093.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28416.1; -; Genomic_DNA.
DR EMBL; AY091076; AAM13896.1; -; mRNA.
DR EMBL; AY122947; AAM67480.1; -; mRNA.
DR EMBL; AK318900; BAH57015.1; -; mRNA.
DR PIR; B86225; B86225.
DR RefSeq; NP_172394.1; NM_100793.4. [Q8RWV8-1]
DR AlphaFoldDB; Q8RWV8; -.
DR SMR; Q8RWV8; -.
DR BioGRID; 22684; 1.
DR IntAct; Q8RWV8; 1.
DR STRING; 3702.AT1G09230.1; -.
DR iPTMnet; Q8RWV8; -.
DR PaxDb; Q8RWV8; -.
DR PRIDE; Q8RWV8; -.
DR EnsemblPlants; AT1G09230.1; AT1G09230.1; AT1G09230. [Q8RWV8-1]
DR GeneID; 837443; -.
DR Gramene; AT1G09230.1; AT1G09230.1; AT1G09230. [Q8RWV8-1]
DR KEGG; ath:AT1G09230; -.
DR Araport; AT1G09230; -.
DR TAIR; locus:2195311; AT1G09230.
DR eggNOG; KOG4206; Eukaryota.
DR HOGENOM; CLU_039888_2_1_1; -.
DR InParanoid; Q8RWV8; -.
DR OMA; RPINTKK; -.
DR PhylomeDB; Q8RWV8; -.
DR PRO; PR:Q8RWV8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RWV8; baseline and differential.
DR Genevisible; Q8RWV8; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005689; C:U12-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IDA:TAIR.
DR GO; GO:0030626; F:U12 snRNA binding; IDA:UniProtKB.
DR GO; GO:0010229; P:inflorescence development; IMP:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IDA:UniProtKB.
DR CDD; cd12238; RRM1_RBM40_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034147; RBM40_RRM1.
DR InterPro; IPR045164; RBM41/RNPC3.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR16105; PTHR16105; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..442
FT /note="U11/U12 small nuclear ribonucleoprotein 65 kDa
FT protein"
FT /id="PRO_0000429831"
FT DOMAIN 28..102
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 352..434
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 101..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 115..136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 373..375
FT /note="GSQ -> AFM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_055303"
FT VAR_SEQ 376..442
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_055304"
SQ SEQUENCE 442 AA; 48712 MW; 9244B871791B731A CRC64;
MAAHATSEPA VNLPATQFES QVTEPFGVTL LVRHLPDGIP HDIVSRLFSQ YGASAVRPCS
GGKLRNAAFV DFKNEAFASQ AHRQLNGLRF LGKVLQVQRA NKPNDNKKSR QIEESVTKGN
AFSTVSTNND SKSGQILSGE PIAPKLGIDY PFPPHLQYAY PPPDANILAN ITNALIAVPP
LYTQVLHLMN KMNLPPPFRL ALPTPPLPKA GPQQTDLEHQ SSSESEMESD EDIGTSKSGR
KRARHGFLVG LGMDKDVPHE TVGVKPSSLT PKEIPRIRKN KHVMQIKITS KVTQDEYKEE
SENEDPADEP KEKDSNLKPF ASLEELEKGR LPPQDILSLP MFKNYTAGNP SVVLYIKNLA
KDVVIDDFYY IFGSQFESSE VAKSSLGVRL MQEGRMRGQA FLTFPSVEVA HRALNLVNGF
VFKGKPMIIQ FGRTPGAAKP NE
