ID   U15_DORVU               Reviewed;          36 AA.
AC   P0DUS4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=U-limacoditoxin(1)-Dv5 {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(1)-Dv5 {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-34, FUNCTION,
RP   SUBCELLULAR LOCATION, PYROGLUTAMATE FORMATION AT GLN-26, AMIDATION AT
RP   TYR-34, SYNTHESIS OF 26-34, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Probable toxin. Does not show activity on the ACP receptor
CC       (ACPR) from the mosquito A.aegypti, on receptors of the closely related
CC       neuropeptides adipokinetic hormone and corazonin. In vivo, does not
CC       reveal any observable effects when injected into crickets
CC       (A.domesticus). Does not induce increase in intracellular calcium in
CC       mouse DRG neurons, suggesting that it does not induce pain.
CC       {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- SIMILARITY: Belongs to the limacoditoxin-1 (ACP-like) family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Pyrrolidone carboxylic acid;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         26..34
FT                   /note="U-limacoditoxin(1)-Dv5"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453400"
FT   MOD_RES         26
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   MOD_RES         34
FT                   /note="Tyrosine amide"
FT                   /evidence="ECO:0000269|PubMed:33893140"
SQ   SEQUENCE   36 AA;  4042 MW;  487DB90047861E0A CRC64;
     MSKLLVLFVV SAMALLQFSM VEATPQKFHV NGRYGK