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C7342_ORYSJ
ID   C7342_ORYSJ             Reviewed;         557 AA.
AC   Q6Z6D6; A0A0P0VG55;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Cytochrome P450 734A2;
DE            EC=1.14.-.-;
GN   Name=CYP734A2; OrderedLocusNames=Os02g0204700, LOC_Os02g11020;
GN   ORFNames=OSJNBb0056C19.10, P0544H11.26;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=21418356; DOI=10.1111/j.1365-313x.2011.04567.x;
RA   Sakamoto T., Kawabe A., Tokida-Segawa A., Shimizu B.I., Takatsuto S.,
RA   Shimada Y., Fujioka S., Mizutani M.;
RT   "Rice CYP734As function as multisubstrate and multifunctional enzymes in
RT   brassinosteroid catabolism.";
RL   Plant J. 67:1-12(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Cytochrome P450 involved in brassinosteroids (BRs)
CC       inactivation and regulation of BRs homeostasis. Is a multifunctional
CC       and multisubstrate enzyme that controls the endogenous bioactive BR
CC       content both by direct inactivation of castasterone (CS) and by
CC       decreasing the levels of BR precursors. Catalyzes the oxidation of
CC       carbon 22 hydroxylated BR intermediates to produce C26 oxidized
CC       metabolites. {ECO:0000269|PubMed:21418356}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, shoot apex, leaf sheaths and
CC       leaf blades. {ECO:0000269|PubMed:21418356}.
CC   -!- INDUCTION: By brassinolide (BL) and dark treatment.
CC       {ECO:0000269|PubMed:21418356}.
CC   -!- MISCELLANEOUS: Plants overexpressing CYP734A2 show a dwarf phenotype,
CC       characterized by abnormal leaves with stiff, tortuous blades,
CC       undeveloped leaf sheaths, no flowering and bear seeds. Over-expression
CC       of CYP734A2 causes an important reduction of the levels of the BRs
CC       castasterone (CS), 6-deoxocastasterone (6-deoxoCS) and 6-
CC       deoxotyphasterol (6-deoxoTY).
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AB488666; BAH23799.1; -; mRNA.
DR   EMBL; AP005008; BAD16010.1; -; Genomic_DNA.
DR   EMBL; AP005915; BAD38430.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAH91576.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS77550.1; -; Genomic_DNA.
DR   RefSeq; XP_015623670.1; XM_015768184.1.
DR   AlphaFoldDB; Q6Z6D6; -.
DR   SMR; Q6Z6D6; -.
DR   STRING; 4530.OS02T0204700-00; -.
DR   PaxDb; Q6Z6D6; -.
DR   PRIDE; Q6Z6D6; -.
DR   EnsemblPlants; Os02t0204700-00; Os02t0204700-00; Os02g0204700.
DR   GeneID; 9268939; -.
DR   Gramene; Os02t0204700-00; Os02t0204700-00; Os02g0204700.
DR   KEGG; osa:9268939; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_001570_5_0_1; -.
DR   InParanoid; Q6Z6D6; -.
DR   OMA; QRFEIRT; -.
DR   OrthoDB; 1247045at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6Z6D6; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IDA:UniProtKB.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0010268; P:brassinosteroid homeostasis; IDA:UniProtKB.
DR   GO; GO:0016131; P:brassinosteroid metabolic process; IDA:UniProtKB.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Growth regulation; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..557
FT                   /note="Cytochrome P450 734A2"
FT                   /id="PRO_0000411201"
FT   TRANSMEM        13..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         495
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   557 AA;  61964 MW;  294621C790EDF9C1 CRC64;
     MEEDGGGGAG WGWATWRVAA VAAAAAVWVT MHVAARMADA LWWRPRRLEA HFAAQGVRGP
     PYRFLLGSVR EMVALMAEAS SKPMSPPTSH NALPRVLAFY HYWRKIYGHR FLIWFGPTPR
     LTVAEPELIR EIFLTRADAF DRYEAHPVVR QLEGDGLVSL HGDKWALHRR VLTDAFYPDN
     LNRLIPHVGK SVAALAAKWG AMAEAGGSGE VEVDVAEWFQ AVTEEAITRA TFGRSYDDGR
     VVFAMQGQLM AFASEAFRKV LVPGYRFLPT KKNRLSWRLD REIRRSLMRL IGRRSDEAEQ
     GEKADDGSFR DLLGLMINAG AAAATRGNAG GEKNSPAAAI PVEDMLEECK TFFFAGKQTT
     TNLLTWATVL LAMHPDWQER ARREVFDVCG AGELPSKEHL PKLKTLGMIM NETLRLYPPA
     VATIRRAKVD VQLSDGCMIP RDMELLVPIM AIHHDTRYWG PDASQFNPAR FANGASKAAK
     HPLAFIPFGL GSRMCVGQNL ARLEAKLTMA ILLQRFEIRT SPNYVHAPTV LMLLYPQYGA
     PLIFRPLSSH PPDSTGP
 
 
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