C7344_ORYSJ
ID C7344_ORYSJ Reviewed; 538 AA.
AC Q69XM6; A0A0P0WYF2; B9X285;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cytochrome P450 734A4;
DE EC=1.14.-.-;
GN Name=CYP734A4; OrderedLocusNames=Os06g0600400, LOC_Os06g39880;
GN ORFNames=OsJ_21885, P0457B11.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=21418356; DOI=10.1111/j.1365-313x.2011.04567.x;
RA Sakamoto T., Kawabe A., Tokida-Segawa A., Shimizu B.I., Takatsuto S.,
RA Shimada Y., Fujioka S., Mizutani M.;
RT "Rice CYP734As function as multisubstrate and multifunctional enzymes in
RT brassinosteroid catabolism.";
RL Plant J. 67:1-12(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Cytochrome P450 involved in brassinosteroids (BRs)
CC inactivation and regulation of BRs homeostasis. Is a multifunctional
CC and multisubstrate enzyme that controls the endogenous bioactive BR
CC content both by direct inactivation of castasterone (CS) and by
CC decreasing the levels of BR precursors. Catalyzes the oxidation of
CC carbon 22 hydroxylated BR intermediates to produce C26 oxidized
CC metabolites. {ECO:0000269|PubMed:21418356}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoot apex, leaf sheaths, leaf
CC blades, internodes and panicles. {ECO:0000269|PubMed:21418356}.
CC -!- INDUCTION: By brassinolide (BL) and dark treatment.
CC {ECO:0000269|PubMed:21418356}.
CC -!- MISCELLANEOUS: Plants overexpressing CYP734A4 show a dwarf phenotype,
CC characterized by abnormal leaves with stiff, tortuous blades,
CC undeveloped leaf sheaths, no flowering and bear seeds. Over-expression
CC of CYP734A4 causes an important reduction of the levels of the BRs
CC castasterone (CS), 6-deoxocastasterone (6-deoxoCS) and 6-
CC deoxotyphasterol (6-deoxoTY).
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AB488667; BAH23800.1; -; mRNA.
DR EMBL; AP003612; BAD32835.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19914.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS98490.1; -; Genomic_DNA.
DR EMBL; CM000143; EEE65973.1; -; Genomic_DNA.
DR RefSeq; XP_015641577.1; XM_015786091.1.
DR AlphaFoldDB; Q69XM6; -.
DR SMR; Q69XM6; -.
DR STRING; 4530.OS06T0600400-01; -.
DR PaxDb; Q69XM6; -.
DR PRIDE; Q69XM6; -.
DR EnsemblPlants; Os06t0600400-01; Os06t0600400-01; Os06g0600400.
DR GeneID; 4341450; -.
DR Gramene; Os06t0600400-01; Os06t0600400-01; Os06g0600400.
DR KEGG; osa:4341450; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_5_0_1; -.
DR InParanoid; Q69XM6; -.
DR OMA; NWAGRFA; -.
DR OrthoDB; 825914at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q69XM6; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IDA:UniProtKB.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IDA:UniProtKB.
DR GO; GO:0016131; P:brassinosteroid metabolic process; IDA:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Growth regulation; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..538
FT /note="Cytochrome P450 734A4"
FT /id="PRO_0000411202"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 480
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 109
FT /note="E -> D (in Ref. 1; BAH23800)"
FT /evidence="ECO:0000305"
FT CONFLICT 352
FT /note="D -> G (in Ref. 1; BAH23800)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 59680 MW; 02AA8F06F4FB01B3 CRC64;
MMEAVAVAAA VLLLLHVAAR VADAVWWRPR RLEAHFAGQG VRGPPYRFLV GCVREMVALM
AEATAKPMPP AAPHNALPRV LAFYHYWRKI YGPTFLIWFG PTPRLTVAEP EMVREIFLTR
AEAFDRYEAH PVVRQLEGDG LVSLHGDKWA HHRRVLTPGF YPDNLNRLVP HVGRSVAALA
ERWRAMACAG GGEVEVDVAE WFQAVAEEAI TRATFGRSYD SGRVVFRLQA RLMAFASEAF
RKVLVPGYRF LPTKKNRMSW GLDREIRRGL VRLIGRRSGG DGGEEDETTT ELKDKQDSGF
NDLLGLMINA GVDRTMPVED MVEECKTFFF AGKQTTTNLL TWATVLLAMH PDWQDRARRE
VLAVCGDAAG ELPTKDHLPK LKTLGMILNE TLRLYPPAVA TIRRAKFDVT LGGGGDGDAG
GIHIPRDTEL LVPIMAIHHD ARLWGPDAAQ FNPARFASGA ARAAKHPLAF IPFGLGSRMC
IGQSLAILEA KLTMAVLLQR FDLALSPTYV HAPTVLMLLH PQYGAPLIFR PRQSQPSN
