U1SBP_HUMAN
ID U1SBP_HUMAN Reviewed; 246 AA.
AC Q16560; A8K262; Q5XKN9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=U11/U12 small nuclear ribonucleoprotein 35 kDa protein;
DE Short=U11/U12 snRNP 35 kDa protein;
DE Short=U11/U12-35K;
DE AltName: Full=Protein HM-1;
DE AltName: Full=U1 snRNP-binding protein homolog;
GN Name=SNRNP35; Synonyms=HM1, U1SNRNPBP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=10520751; DOI=10.3109/10425179809105207;
RA Adams D.S., Li Q., Tan X., Pero S.C., Czop J.K.;
RT "Cloning and characterization of a family of cDNAs from human histiocyte
RT macrophage cells encoding an arginine-rich basic protein related to the 70
RT kD U1-snRNP splicing factor.";
RL DNA Seq. 9:205-215(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, Lung, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION, IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=15146077; DOI=10.1261/rna.7320604;
RA Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S.,
RA Tuschl T., Luehrmann R.;
RT "The human 18S U11/U12 snRNP contains a set of novel proteins not found in
RT the U2-dependent spliceosome.";
RL RNA 10:929-941(2004).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-172, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- SUBUNIT: Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome. {ECO:0000269|PubMed:15146077}.
CC -!- INTERACTION:
CC Q16560-2; X5D778: ANKRD11; NbExp=5; IntAct=EBI-12938570, EBI-17183751;
CC Q16560-2; P45973: CBX5; NbExp=3; IntAct=EBI-12938570, EBI-78219;
CC Q16560-2; Q2TAC2-2: CCDC57; NbExp=3; IntAct=EBI-12938570, EBI-10961624;
CC Q16560-2; P40227: CCT6A; NbExp=3; IntAct=EBI-12938570, EBI-356687;
CC Q16560-2; P61024: CKS1B; NbExp=3; IntAct=EBI-12938570, EBI-456371;
CC Q16560-2; P49761: CLK3; NbExp=3; IntAct=EBI-12938570, EBI-745579;
CC Q16560-2; Q9H8Y8: GORASP2; NbExp=3; IntAct=EBI-12938570, EBI-739467;
CC Q16560-2; Q9H8W4: PLEKHF2; NbExp=3; IntAct=EBI-12938570, EBI-742388;
CC Q16560-2; P17612: PRKACA; NbExp=3; IntAct=EBI-12938570, EBI-476586;
CC Q16560-2; P20340-2: RAB6A; NbExp=3; IntAct=EBI-12938570, EBI-8840191;
CC Q16560-2; P0C264: SBK3; NbExp=3; IntAct=EBI-12938570, EBI-17181801;
CC Q16560-2; P50454: SERPINH1; NbExp=3; IntAct=EBI-12938570, EBI-350723;
CC Q16560-2; Q92966: SNAPC3; NbExp=3; IntAct=EBI-12938570, EBI-1760638;
CC Q16560-2; Q9BSW7: SYT17; NbExp=3; IntAct=EBI-12938570, EBI-745392;
CC Q16560-2; P37173: TGFBR2; NbExp=3; IntAct=EBI-12938570, EBI-296151;
CC Q16560-2; Q01081: U2AF1; NbExp=3; IntAct=EBI-12938570, EBI-632461;
CC Q16560-2; P55072: VCP; NbExp=3; IntAct=EBI-12938570, EBI-355164;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15146077}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16560-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16560-2; Sequence=VSP_028855;
CC -!- TISSUE SPECIFICITY: Expressed in heart, liver, skeletal muscle and
CC pancreas. {ECO:0000269|PubMed:10520751}.
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DR EMBL; U44798; AAA86654.1; -; mRNA.
DR EMBL; U44799; AAA86655.1; -; mRNA.
DR EMBL; AK290127; BAF82816.1; -; mRNA.
DR EMBL; CH471054; EAW98412.1; -; Genomic_DNA.
DR EMBL; BC009622; AAH09622.1; -; mRNA.
DR EMBL; BC047678; AAH47678.1; -; mRNA.
DR EMBL; BC054034; AAH54034.1; -; mRNA.
DR EMBL; BC093671; AAH93671.1; -; mRNA.
DR EMBL; BC111955; AAI11956.1; -; mRNA.
DR EMBL; BK005197; DAA05495.1; -; mRNA.
DR CCDS; CCDS45005.1; -. [Q16560-2]
DR CCDS; CCDS9249.1; -. [Q16560-1]
DR PIR; G02371; G02371.
DR RefSeq; NP_073208.1; NM_022717.3. [Q16560-1]
DR RefSeq; NP_851030.1; NM_180699.3. [Q16560-2]
DR RefSeq; XP_006719267.1; XM_006719204.3. [Q16560-1]
DR RefSeq; XP_016874213.1; XM_017018724.1. [Q16560-2]
DR AlphaFoldDB; Q16560; -.
DR SMR; Q16560; -.
DR BioGRID; 116250; 34.
DR CORUM; Q16560; -.
DR IntAct; Q16560; 32.
DR MINT; Q16560; -.
DR STRING; 9606.ENSP00000403310; -.
DR iPTMnet; Q16560; -.
DR PhosphoSitePlus; Q16560; -.
DR BioMuta; SNRNP35; -.
DR DMDM; 74754517; -.
DR EPD; Q16560; -.
DR jPOST; Q16560; -.
DR MassIVE; Q16560; -.
DR MaxQB; Q16560; -.
DR PaxDb; Q16560; -.
DR PeptideAtlas; Q16560; -.
DR PRIDE; Q16560; -.
DR ProteomicsDB; 60917; -. [Q16560-1]
DR ProteomicsDB; 60918; -. [Q16560-2]
DR Antibodypedia; 31812; 51 antibodies from 17 providers.
DR DNASU; 11066; -.
DR Ensembl; ENST00000350887.5; ENSP00000340774.5; ENSG00000184209.15. [Q16560-1]
DR Ensembl; ENST00000412157.2; ENSP00000403310.2; ENSG00000184209.15. [Q16560-2]
DR Ensembl; ENST00000526639.3; ENSP00000432595.2; ENSG00000184209.15. [Q16560-1]
DR GeneID; 11066; -.
DR KEGG; hsa:11066; -.
DR MANE-Select; ENST00000526639.3; ENSP00000432595.2; NM_022717.4; NP_073208.1.
DR UCSC; uc001ufb.2; human. [Q16560-1]
DR CTD; 11066; -.
DR DisGeNET; 11066; -.
DR GeneCards; SNRNP35; -.
DR HGNC; HGNC:30852; SNRNP35.
DR HPA; ENSG00000184209; Low tissue specificity.
DR MIM; 619631; gene.
DR neXtProt; NX_Q16560; -.
DR OpenTargets; ENSG00000184209; -.
DR PharmGKB; PA164726115; -.
DR VEuPathDB; HostDB:ENSG00000184209; -.
DR eggNOG; KOG0113; Eukaryota.
DR GeneTree; ENSGT00940000157648; -.
DR HOGENOM; CLU_035088_1_0_1; -.
DR InParanoid; Q16560; -.
DR OMA; FEHERDF; -.
DR OrthoDB; 1430110at2759; -.
DR PhylomeDB; Q16560; -.
DR TreeFam; TF331614; -.
DR PathwayCommons; Q16560; -.
DR Reactome; R-HSA-72165; mRNA Splicing - Minor Pathway.
DR SignaLink; Q16560; -.
DR BioGRID-ORCS; 11066; 728 hits in 1087 CRISPR screens.
DR ChiTaRS; SNRNP35; human.
DR GenomeRNAi; 11066; -.
DR Pharos; Q16560; Tdark.
DR PRO; PR:Q16560; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q16560; protein.
DR Bgee; ENSG00000184209; Expressed in tendon of biceps brachii and 202 other tissues.
DR ExpressionAtlas; Q16560; baseline and differential.
DR Genevisible; Q16560; HS.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005689; C:U12-type spliceosomal complex; IDA:HGNC-UCL.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0017069; F:snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0008380; P:RNA splicing; IC:HGNC-UCL.
DR CDD; cd12237; RRM_snRNP35; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034146; snRNP35_RRM.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isopeptide bond; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; RNA-binding; Spliceosome; Ubl conjugation.
FT CHAIN 1..246
FT /note="U11/U12 small nuclear ribonucleoprotein 35 kDa
FT protein"
FT /id="PRO_0000307907"
FT DOMAIN 51..129
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 187..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 172
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1
FT /note="M -> MKPANM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028855"
SQ SEQUENCE 246 AA; 29450 MW; E420E6D719D3B0C7 CRC64;
MNDWMPIAKE YDPLKAGSID GTDEDPHDRA VWRAMLARYV PNKGVIGDPL LTLFVARLNL
QTKEDKLKEV FSRYGDIRRL RLVRDLVTGF SKGYAFIEYK EERAVIKAYR DADGLVIDQH
EIFVDYELER TLKGWIPRRL GGGLGGKKES GQLRFGGRDR PFRKPINLPV VKNDLYREGK
RERRERSRSR ERHWDSRTRD RDHDRGREKR WQEREPTRVW PDNDWERERD FRDDRIKGRE
KKERGK
