C7345_ORYSJ
ID C7345_ORYSJ Reviewed; 537 AA.
AC Q8LIF2; B9X286;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cytochrome P450 734A5;
DE EC=1.14.-.-;
GN Name=CYP734A5; OrderedLocusNames=Os07g0647200, LOC_Os07g45290;
GN ORFNames=OJ1316_A04.108, OsJ_25362, P0503D09.125;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=21418356; DOI=10.1111/j.1365-313x.2011.04567.x;
RA Sakamoto T., Kawabe A., Tokida-Segawa A., Shimizu B.I., Takatsuto S.,
RA Shimada Y., Fujioka S., Mizutani M.;
RT "Rice CYP734As function as multisubstrate and multifunctional enzymes in
RT brassinosteroid catabolism.";
RL Plant J. 67:1-12(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Cytochrome P450 probably involved in brassinosteroids (BRs)
CC inactivation and regulation of BRs homeostasis. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Exclusively expressed in roots.
CC {ECO:0000269|PubMed:21418356}.
CC -!- INDUCTION: By brassinolide and dark treatment.
CC {ECO:0000269|PubMed:21418356}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAH23801.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AB488668; BAH23801.1; ALT_SEQ; mRNA.
DR EMBL; AP003822; BAC06993.1; -; Genomic_DNA.
DR EMBL; AP005455; BAD31455.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF22381.1; -; Genomic_DNA.
DR EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000144; EAZ40884.1; -; Genomic_DNA.
DR RefSeq; XP_015648047.1; XM_015792561.1.
DR AlphaFoldDB; Q8LIF2; -.
DR SMR; Q8LIF2; -.
DR STRING; 4530.OS07T0647200-00; -.
DR PaxDb; Q8LIF2; -.
DR PRIDE; Q8LIF2; -.
DR GeneID; 4344107; -.
DR KEGG; osa:4344107; -.
DR eggNOG; KOG0157; Eukaryota.
DR InParanoid; Q8LIF2; -.
DR OrthoDB; 467733at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR GO; GO:0016131; P:brassinosteroid metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..537
FT /note="Cytochrome P450 734A5"
FT /id="PRO_0000411203"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 480
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 537 AA; 59910 MW; 068441D7D046ED20 CRC64;
MWSSWAWTWS WSGAAAVAVA AAAAWVAVYA AAARAAEALW WRPRRVERHF AAQGVRGPGY
RFFVGSSIEL VRLMVDAASR PMEPPTSHDI LPRVLPFYHH WRKLYGPMHL IWFGRTPRLV
VSEPELIREV LLTRADHFDR YEAHPMICQF EGYGLSNLHG ERWARRRRVL TPAFHTENLR
MIAPFVAGTV TRMLDELAER ARAGGAGEAE VDVAEWFQRV PQEAITFAAF GRRNYDDGAA
VFRLQDELAG YATEAHSKVY IPGYRFLPTR KNRRVWQLDR EIRSHLAKFV TGLQSCSSSH
GDDADDGGDG GGGMREFMSF MAPAMTAGEI IEESKNFFFA GKETLSNLLT WTTVALAMHP
EWQERARREV VAVCGRGDLP TKDHLPKLKT LGMILNETLR LYPPAVAMIR TAKEDVELGG
CVVPAGTEVM IPIMAVHHDA AAWGDDAAEF NPARFAADDD GGRRRHPMAF MPFGGGARVC
IGQNMALMEA KVALAVVLRR FEFRLSPAYV HAPRVLMILS PQFGAPVIFR PLTSAAA
