U1_BPMU
ID U1_BPMU Reviewed; 175 AA.
AC Q9T1U9;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 23-FEB-2022, entry version 62.
DE RecName: Full=Tail fiber assembly protein U;
DE AltName: Full=Gene product 50;
DE Short=gp50;
DE AltName: Full=Gene product U;
DE Short=gpU;
GN Name=U; OrderedLocusNames=Mup50;
OS Escherichia phage Mu (Bacteriophage Mu).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Muvirus.
OX NCBI_TaxID=10677;
OH NCBI_TaxID=543; Enterobacteriaceae.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT prophages in Haemophilus, Neisseria and Deinococcus.";
RL J. Mol. Biol. 317:337-359(2002).
RN [2]
RP DISRUPTION PHENOTYPE.
RX PubMed=3904174; DOI=10.1016/0042-6822(85)90388-5;
RA Grundy F.J., Howe M.M.;
RT "Morphogenetic structures present in lysates of amber mutants of
RT bacteriophage Mu.";
RL Virology 143:485-504(1985).
RN [3]
RP INDUCTION.
RX PubMed=8293968; DOI=10.1093/genetics/135.3.619;
RA Chiang L.W., Howe M.M.;
RT "Mutational analysis of a C-dependent late promoter of bacteriophage Mu.";
RL Genetics 135:619-629(1993).
CC -!- FUNCTION: Chaperone involved in tail fiber assembly. Remains associated
CC to the tail fiber and participates in the host receptor binding. Binds
CC to the primary receptor (Probable). Two alternate tail fiber assembly
CC proteins U and U' are encoded extending the host range of the virus.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000305}. Note=Tail
CC fiber.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC Expression of late genes is activated by the viral late transcription
CC activator C. Expressed alternatively with tail fiber assembly protein
CC U'. The switch from S-U to S'-U' is performed through inversion of a
CC DNA segment called G by the phage invertase protein Gin.
CC {ECO:0000269|PubMed:8293968}.
CC -!- DISRUPTION PHENOTYPE: Viral particles lack tail fibers in inversion-
CC defective gin mutants (blocked in G+ orientation; no switch to S'-U').
CC {ECO:0000269|PubMed:3904174}.
CC -!- MISCELLANEOUS: The orientation of the G segment is defined as G+ and
CC G-. G+ orientation provides S-U fibers whereas G- provides S'-U'
CC fibers. S-U and S'-U' dont have the same host range (e.g. respectively
CC E.coli and C.freundii).
CC -!- SIMILARITY: Belongs to the tfa family. {ECO:0000305}.
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DR EMBL; AF083977; AAF01128.1; -; Genomic_DNA.
DR RefSeq; NP_050654.1; NC_000929.1.
DR PDB; 5YVQ; X-ray; 2.10 A; B=1-175.
DR PDBsum; 5YVQ; -.
DR SMR; Q9T1U9; -.
DR GeneID; 2636270; -.
DR KEGG; vg:2636270; -.
DR Proteomes; UP000002611; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0098024; C:virus tail, fiber; IEA:UniProtKB-KW.
DR GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0098678; P:viral tropism switching; IEA:UniProtKB-KW.
DR GO; GO:0098004; P:virus tail fiber assembly; IEA:UniProtKB-KW.
DR InterPro; IPR003458; Phage_T4_Gp38_tail_assem.
DR Pfam; PF02413; Caudo_TAP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Host cytoplasm; Host-virus interaction;
KW Late protein; Reference proteome;
KW Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW Viral receptor tropism switching; Viral release from host cell;
KW Viral tail assembly; Viral tail fiber assembly; Viral tail fiber protein;
KW Viral tail protein; Virion; Virus entry into host cell.
FT CHAIN 1..175
FT /note="Tail fiber assembly protein U"
FT /id="PRO_0000070311"
FT HELIX 15..24
FT /evidence="ECO:0007829|PDB:5YVQ"
FT HELIX 37..40
FT /evidence="ECO:0007829|PDB:5YVQ"
FT HELIX 41..43
FT /evidence="ECO:0007829|PDB:5YVQ"
FT STRAND 48..53
FT /evidence="ECO:0007829|PDB:5YVQ"
FT STRAND 58..64
FT /evidence="ECO:0007829|PDB:5YVQ"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:5YVQ"
FT STRAND 74..79
FT /evidence="ECO:0007829|PDB:5YVQ"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:5YVQ"
FT STRAND 94..102
FT /evidence="ECO:0007829|PDB:5YVQ"
SQ SEQUENCE 175 AA; 20310 MW; 71A0EBA3294FCCD1 CRC64;
MMHLKNIKSE NPKTKEQYQL TKNFDVIWLW SEDGKNWYEE VNNFQDDTIK IVYDENNIIV
AITKDASTLN PEGFSVVEIP DITANRRADD SGKWMFKDGA VVKRIYTADE QQQQAESQKA
ALLSEAESVI QPLERAVRLN MATDEERARL ESWERYSVLV SRVDTANPEW PQKPE
