C7346_ORYSJ
ID C7346_ORYSJ Reviewed; 542 AA.
AC B9X287; Q0JML1; Q5VMP8;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Cytochrome P450 734A6;
DE EC=1.14.-.-;
GN Name=CYP734A6; OrderedLocusNames=Os01g0388000, LOC_Os01g29150;
GN ORFNames=OSJNBb0008D07.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=21418356; DOI=10.1111/j.1365-313x.2011.04567.x;
RA Sakamoto T., Kawabe A., Tokida-Segawa A., Shimizu B.I., Takatsuto S.,
RA Shimada Y., Fujioka S., Mizutani M.;
RT "Rice CYP734As function as multisubstrate and multifunctional enzymes in
RT brassinosteroid catabolism.";
RL Plant J. 67:1-12(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Cytochrome P450 involved in brassinosteroids (BRs)
CC inactivation and regulation of BRs homeostasis. Is a multifunctional
CC and multisubstrate enzyme that controls the endogenous bioactive BR
CC content both by direct inactivation of castasterone (CS) and by
CC decreasing the levels of BR precursors. Catalyzes the oxidation of
CC carbon 22 hydroxylated BR intermediates to produce C26 oxidized
CC metabolites. {ECO:0000269|PubMed:21418356}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaf sheaths. Expressed in
CC roots, shoot apex, leaf blades, internodes and panicles.
CC {ECO:0000269|PubMed:21418356}.
CC -!- INDUCTION: By brassinolide (BL). {ECO:0000269|PubMed:21418356}.
CC -!- MISCELLANEOUS: Plants overexpressing CYP734A6 show a dwarf phenotype,
CC characterized by abnormal leaves with stiff, tortuous blades,
CC undeveloped leaf sheaths, no flowering and bear seeds. Over-expression
CC of CYP734A6 causes an important reduction of the levels of the BRs
CC castasterone (CS), 6-deoxocastasterone (6-deoxoCS) and 6-
CC deoxotyphasterol (6-deoxoTY).
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD69277.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF05017.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB488669; BAH23802.1; -; mRNA.
DR EMBL; AP006237; BAD69277.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAF05017.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015631278.1; XM_015775792.1.
DR AlphaFoldDB; B9X287; -.
DR SMR; B9X287; -.
DR STRING; 4530.OS01T0388000-01; -.
DR PRIDE; B9X287; -.
DR GeneID; 4325222; -.
DR KEGG; osa:4325222; -.
DR InParanoid; B9X287; -.
DR OrthoDB; 825914at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IDA:UniProtKB.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IDA:UniProtKB.
DR GO; GO:0016131; P:brassinosteroid metabolic process; IDA:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Growth regulation; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..542
FT /note="Cytochrome P450 734A6"
FT /id="PRO_0000411204"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 474
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 542 AA; 60803 MW; 64A3E7780DD59442 CRC64;
MGWWGWAAAA AAAAAWVAVK VLEVLWWRPR RVEEHFARQG ITGPRYRFLV GCVREMVALM
VAASAKPMPP PYRSHNVLPR VLAFYHHWKK IYGSTFLIWF GPTPRLAIAD PELIREVLLA
RADRFDRYES HPMVRQLEGE GLVSLRGDKW AHHRRVLTPA FHMDNLRLLL PCVGMTVLDM
ADKWRAMAEA DKSGEVEIDV SDWFQVVTED AITRTAFGRS YEDGKVVFKL QAQLMAFASE
AFRKVFIPGY RFLPTKKNTS SWKLDKEIRK NLVTLIGRRQ EAGDDEKLDG CAKDLLGLMI
NAAASSNGGK RSALPVSPIT VNDIVEECKT FFFAGKQTTS NLLTWAIVVL AMHPEWQERA
RQEVLDVCGA DGVPSREQLA KLKTLGMILN ETLRLYPPAV ATVRRAKADV ELGGYLRIPR
DTELLIPIMA VHHDARLWGP DAAQFNPARF AGGVARAARH PAAFIPFGLG ARMCIGQNLA
ILEAKLTVAV ILHRFEFRLS ARYVHAPTVL MLLHPQYGAP IVFRPRSSSQ PTCEKMNPLT
SS
