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C734A_ARATH
ID   C734A_ARATH             Reviewed;         520 AA.
AC   O48786;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=Cytochrome P450 734A1;
DE            EC=1.14.-.-;
DE   AltName: Full=Protein PHYB ACTIVATION-TAGGED SUPPRESSOR 1;
GN   Name=CYP734A1; Synonyms=BAS1; OrderedLocusNames=At2g26710;
GN   ORFNames=F18A8.8;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION.
RX   PubMed=10611382; DOI=10.1073/pnas.96.26.15316;
RA   Neff M.M., Nguyen S.M., Malancharuvil E.J., Fujioka S., Noguchi T.,
RA   Seto H., Tsubuki M., Honda T., Takatsuto S., Yoshida S., Chory J.;
RT   "BAS1: A gene regulating brassinosteroid levels and light responsiveness in
RT   Arabidopsis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:15316-15323(1999).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Wassilewskija-2;
RX   PubMed=14605216; DOI=10.1104/pp.103.030882;
RA   Turk E.M., Fujioka S., Seto H., Shimada Y., Takatsuto S., Yoshida S.,
RA   Denzel M.A., Torres Q.I., Neff M.M.;
RT   "CYP72B1 inactivates brassinosteroid hormones: an intersection between
RT   photomorphogenesis and plant steroid signal transduction.";
RL   Plant Physiol. 133:1643-1653(2003).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=15773851; DOI=10.1111/j.1365-313x.2005.02358.x;
RA   Turk E.M., Fujioka S., Seto H., Shimada Y., Takatsuto S., Yoshida S.,
RA   Wang H., Torres Q.I., Ward J.M., Murthy G., Zhang J., Walker J.C.,
RA   Neff M.M.;
RT   "BAS1 and SOB7 act redundantly to modulate Arabidopsis photomorphogenesis
RT   via unique brassinosteroid inactivation mechanisms.";
RL   Plant J. 42:23-34(2005).
RN   [8]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=20396969; DOI=10.1007/s10059-010-0055-6;
RA   Jung J.H., Lee M., Park C.M.;
RT   "A transcriptional feedback loop modulating signaling crosstalks between
RT   auxin and brassinosteroid in Arabidopsis.";
RL   Mol. Cells 29:449-456(2010).
RN   [9]
RP   FUNCTION.
RX   PubMed=20669042; DOI=10.1007/s11103-010-9663-y;
RA   Thornton L.E., Rupasinghe S.G., Peng H., Schuler M.A., Neff M.M.;
RT   "Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates
RT   brassinosteroids.";
RL   Plant Mol. Biol. 74:167-181(2010).
CC   -!- FUNCTION: Cytochrome P450 involved in brassinosteroids (BRs)
CC       inactivation and regulation of BRs homeostasis. Inactivates the BRs
CC       castasterone (CS) and brassinolide (BL) through carbon 26
CC       hydroxylation. Acts in association with CYP72C1 to inactivate BRs and
CC       modulate photomorphogenesis. {ECO:0000269|PubMed:10611382,
CC       ECO:0000269|PubMed:14605216, ECO:0000269|PubMed:15773851,
CC       ECO:0000269|PubMed:20396969, ECO:0000269|PubMed:20669042}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: By brassinolide (BL), auxin and dark treatment.
CC       {ECO:0000269|PubMed:14605216, ECO:0000269|PubMed:20396969}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       condition, but reduced responsiveness of hypocotyls to light and
CC       increased responsiveness to brassinolide (BL). Strong reduction of C-26
CC       hydroxylase activity. {ECO:0000269|PubMed:14605216,
CC       ECO:0000269|PubMed:15773851}.
CC   -!- MISCELLANEOUS: Plants overexpressing CYP734A1 exhibit severe dwarfism.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AC003105; AAB95305.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07878.1; -; Genomic_DNA.
DR   EMBL; BT010564; AAQ65187.1; -; mRNA.
DR   EMBL; AK175232; BAD42995.1; -; mRNA.
DR   EMBL; AK229743; BAF01580.1; -; mRNA.
DR   PIR; H84663; H84663.
DR   RefSeq; NP_180239.1; NM_128228.4.
DR   AlphaFoldDB; O48786; -.
DR   SMR; O48786; -.
DR   STRING; 3702.AT2G26710.1; -.
DR   PaxDb; O48786; -.
DR   PRIDE; O48786; -.
DR   ProteomicsDB; 240266; -.
DR   EnsemblPlants; AT2G26710.1; AT2G26710.1; AT2G26710.
DR   GeneID; 817212; -.
DR   Gramene; AT2G26710.1; AT2G26710.1; AT2G26710.
DR   KEGG; ath:AT2G26710; -.
DR   Araport; AT2G26710; -.
DR   TAIR; locus:2043823; AT2G26710.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_001570_5_0_1; -.
DR   InParanoid; O48786; -.
DR   OMA; IAGRTMF; -.
DR   OrthoDB; 825914at2759; -.
DR   PhylomeDB; O48786; -.
DR   PRO; PR:O48786; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O48786; baseline and differential.
DR   Genevisible; O48786; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IMP:TAIR.
DR   GO; GO:0010268; P:brassinosteroid homeostasis; IEP:TAIR.
DR   GO; GO:0016131; P:brassinosteroid metabolic process; IDA:TAIR.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0009741; P:response to brassinosteroid; IEP:TAIR.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Growth regulation; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..520
FT                   /note="Cytochrome P450 734A1"
FT                   /id="PRO_0000411195"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         463
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   520 AA;  59490 MW;  5B51EB6A0CC0FA59 CRC64;
     MEEESSSWFI PKVLVLSVIL SLVIVKGMSL LWWRPRKIEE HFSKQGIRGP PYHFFIGNVK
     ELVGMMLKAS SHPMPFSHNI LPRVLSFYHH WRKIYGATFL VWFGPTFRLT VADPDLIREI
     FSKSEFYEKN EAHPLVKQLE GDGLLSLKGE KWAHHRKIIS PTFHMENLKL LVPVVLKSVT
     DMVDKWSDKL SENGEVEVDV YEWFQILTED VISRTAFGSS YEDGRAVFRL QAQQMLLCAE
     AFQKVFIPGY RFFPTRGNLK SWKLDKEIRK SLLKLIERRR QNAIDGEGEE CKEPAAKDLL
     GLMIQAKNVT VQDIVEECKS FFFAGKQTTS NLLTWTTILL SMHPEWQAKA RDEVLRVCGS
     RDVPTKDHVV KLKTLSMILN ESLRLYPPIV ATIRRAKSDV KLGGYKIPCG TELLIPIIAV
     HHDQAIWGND VNEFNPARFA DGVPRAAKHP VGFIPFGLGV RTCIGQNLAI LQAKLTLAVM
     IQRFTFHLAP TYQHAPTVLM LLYPQHGAPI TFRRLTNHED
 
 
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