ID   U2A2A_NICPL             Reviewed;         555 AA.
AC   Q9ZR39;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Splicing factor U2af large subunit A;
DE   AltName: Full=NpU2AF65a;
DE   AltName: Full=U2 auxiliary factor 65 kDa subunit A;
DE   AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit A;
DE            Short=U2 snRNP auxiliary factor large subunit A;
GN   Name=U2AF65A;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=9852132; DOI=10.1074/jbc.273.51.34603;
RA   Domon C., Lorkovic Z.J., Valcarcel J., Filipowicz W.;
RT   "Multiple forms of the U2 small nuclear ribonucleoprotein auxiliary factor
RT   U2AF subunits expressed in higher plants.";
RL   J. Biol. Chem. 273:34603-34610(1998).
CC   -!- FUNCTION: Necessary for the splicing of pre-mRNA. Binds to the U
CC       -enriched regions of plant introns. {ECO:0000269|PubMed:9852132}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9852132}.
CC   -!- TISSUE SPECIFICITY: Expressed in stems, leaves and apical buds.
CC       {ECO:0000269|PubMed:9852132}.
CC   -!- DOMAIN: N-terminal RS domain has a very strong bias in favor of D over
CC       S.
CC   -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR   EMBL; Y18351; CAA77136.1; -; mRNA.
DR   AlphaFoldDB; Q9ZR39; -.
DR   SMR; Q9ZR39; -.
DR   PRIDE; Q9ZR39; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.330; -; 3.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR003954; RRM_dom_euk.
DR   InterPro; IPR006529; U2AF_lg.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 3.
DR   SMART; SM00361; RRM_1; 2.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR   PROSITE; PS50102; RRM; 3.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Repeat; RNA-binding.
FT   CHAIN           1..555
FT                   /note="Splicing factor U2af large subunit A"
FT                   /id="PRO_0000352269"
FT   DOMAIN          221..304
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          341..419
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          460..546
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..133
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..151
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   555 AA;  62168 MW;  9DF31E5B2A815E8A CRC64;
     MRDYEGNGVD IDNNGASPYS QHISRDHENE RDSSRSRDKE RDKGRDKDRD RDRNRDRDRD
     RDRVKERDKD RHRDRDGEKD RDRHHRDRHR DRSDRRERER TRDRDEDDLH RSRDYDRRRD
     NDKDREDRRR HRPSSRGRSE HRSKSRSRSP SKSKRISGFD MAPPTTALLP GATDAAGQVP
     GTNPAIPGLF SNMFPLASSQ FGALPMMPVQ AMTQQATRHA RRVYVGGLPP TANEQSVATF
     FSHVMYAIGG NTAGPGDAVV NVYINHEKKF AFVEMRSVEE ASNAMALDGV IFEGGPVKVR
     RPSDYNPSLA ATLGPSQPSP NLNLAAVGST PGSSGGLEGP DRIFVGGLPY YFTESQIREL
     LESFGQLRGF DLVKDRETGN SKGYAFCVYQ DVSVTDIACA ALNGIKMGDK TLTVRRANQG
     TTQPNPEQES VLLHAQQQIA LQRFMLQPGA LATKVLCLTE VVTVDELNDD DDYQDILEDM
     RTECEKFGAL VNVVIPRPNP NGVPTPGLGK VFLEYADVDG SSKARQGLNG RKFGGNQVVA
     VFYPENKFSE GDYEA