ID   U2A2A_WHEAT             Reviewed;         591 AA.
AC   Q2QKB3;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Splicing factor U2af large subunit A;
DE   AltName: Full=U2 auxiliary factor 65 kDa subunit A;
DE   AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit A;
DE            Short=U2 snRNP auxiliary factor large subunit A;
GN   Name=U2AF65A;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Chinese Spring; TISSUE=Grain;
RX   PubMed=16941218; DOI=10.1007/s11103-006-9046-6;
RA   Lopato S., Borisjuk L., Milligan A.S., Shirley N., Bazanova N.,
RA   Langridge P.;
RT   "Systematic identification of factors involved in post-transcriptional
RT   processes in wheat grain.";
RL   Plant Mol. Biol. 62:637-653(2006).
CC   -!- FUNCTION: Necessary for the splicing of pre-mRNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: N-terminal RS domain has a very strong bias in favor of D over
CC       S.
CC   -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR   EMBL; DQ019636; AAY84881.1; -; mRNA.
DR   AlphaFoldDB; Q2QKB3; -.
DR   SMR; Q2QKB3; -.
DR   STRING; 4565.Traes_5AL_647E44702.2; -.
DR   PRIDE; Q2QKB3; -.
DR   EnsemblPlants; TraesCAD_scaffold_004691_01G000200.1; TraesCAD_scaffold_004691_01G000200.1; TraesCAD_scaffold_004691_01G000200.
DR   EnsemblPlants; TraesCLE_scaffold_004645_01G000700.1; TraesCLE_scaffold_004645_01G000700.1; TraesCLE_scaffold_004645_01G000700.
DR   EnsemblPlants; TraesPAR_scaffold_004850_01G000500.1; TraesPAR_scaffold_004850_01G000500.1; TraesPAR_scaffold_004850_01G000500.
DR   EnsemblPlants; TraesROB_scaffold_009827_01G000300.1; TraesROB_scaffold_009827_01G000300.1; TraesROB_scaffold_009827_01G000300.
DR   EnsemblPlants; TraesWEE_scaffold_011160_01G000400.1; TraesWEE_scaffold_011160_01G000400.1; TraesWEE_scaffold_011160_01G000400.
DR   Gramene; TraesCAD_scaffold_004691_01G000200.1; TraesCAD_scaffold_004691_01G000200.1; TraesCAD_scaffold_004691_01G000200.
DR   Gramene; TraesCLE_scaffold_004645_01G000700.1; TraesCLE_scaffold_004645_01G000700.1; TraesCLE_scaffold_004645_01G000700.
DR   Gramene; TraesPAR_scaffold_004850_01G000500.1; TraesPAR_scaffold_004850_01G000500.1; TraesPAR_scaffold_004850_01G000500.
DR   Gramene; TraesROB_scaffold_009827_01G000300.1; TraesROB_scaffold_009827_01G000300.1; TraesROB_scaffold_009827_01G000300.
DR   Gramene; TraesWEE_scaffold_011160_01G000400.1; TraesWEE_scaffold_011160_01G000400.1; TraesWEE_scaffold_011160_01G000400.
DR   eggNOG; KOG0120; Eukaryota.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; Q2QKB3; baseline and differential.
DR   GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR   GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR   GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR   GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR   GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR   GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR006529; U2AF_lg.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW   RNA-binding.
FT   CHAIN           1..591
FT                   /note="Splicing factor U2af large subunit A"
FT                   /id="PRO_0000352273"
FT   DOMAIN          272..355
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          392..470
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..215
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..190
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   591 AA;  67945 MW;  5244E144873637C9 CRC64;
     MAEHDAPPES GAARSPPAKK RGGDARSPQQ DAQPLSSRDR VRERDEDKDR ERHRRHGEDR
     ERYRDRESVR ERGEGSRDRE RHRREHREES RDRERHHREH REGSRDRERH HREHREGSRD
     RERHHREHRE GSRDRERHHR DHREGSRDRE RHHRDHRERS ERREHRDRSD DRDYRRSCDR
     DAERRDRDRD GHRRHRSRSP LRSESQSKRM SGFDQRPSEA IPILAPDATP SQLPELPAAN
     PGMFPNMLPN LVNVPALGQP LAMTQQATRH ARRVYVGGLP PIANEQTVAV FFNQVMAAIG
     GNTFALGHAV VNVYINHDKK FAFVEMRSVE EASNAMALDG IMFEGAPVKV RRPTDYNPSQ
     AAALGPSQPN PNLNLAAVGL TPGAGGGLEG PDRIFVGGLP YYFTEAQVRE LLETFGPLRG
     FDIVKDKETG NSKGYAFCLY KDGTVTDIAC AALNGIQLGD RTLTVRRANQ GAEPRPEQEN
     ILLQAQQEAQ MKRLVYEVGR TLTTKVVCLT QVVSADDLRD DEEYNDILED MTLEGHKYVP
     HSTIAESFII RPHAKFAIRP KLTEDTNLFH LDLTNHMFSS LPFCHVHVCS Y