ID   U2A2B_NICPL             Reviewed;         573 AA.
AC   Q9ZR40;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Splicing factor U2af large subunit B;
DE   AltName: Full=NpU2AF65b;
DE   AltName: Full=U2 auxiliary factor 65 kDa subunit B;
DE   AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit B;
DE            Short=U2 snRNP auxiliary factor large subunit B;
GN   Name=U2AF65B;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=9852132; DOI=10.1074/jbc.273.51.34603;
RA   Domon C., Lorkovic Z.J., Valcarcel J., Filipowicz W.;
RT   "Multiple forms of the U2 small nuclear ribonucleoprotein auxiliary factor
RT   U2AF subunits expressed in higher plants.";
RL   J. Biol. Chem. 273:34603-34610(1998).
CC   -!- FUNCTION: Necessary for the splicing of pre-mRNA. Binds to the U
CC       -enriched regions of plant introns. {ECO:0000269|PubMed:9852132}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in stems, leaves and apical buds.
CC       {ECO:0000269|PubMed:9852132}.
CC   -!- DOMAIN: N-terminal RS domain has a very strong bias in favor of D over
CC       S.
CC   -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR   EMBL; Y18350; CAA77135.1; -; mRNA.
DR   AlphaFoldDB; Q9ZR40; -.
DR   SMR; Q9ZR40; -.
DR   PRIDE; Q9ZR40; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.330; -; 3.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR006529; U2AF_lg.
DR   Pfam; PF00076; RRM_1; 2.
DR   SMART; SM00360; RRM; 3.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR   PROSITE; PS50102; RRM; 3.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Repeat; RNA-binding.
FT   CHAIN           1..573
FT                   /note="Splicing factor U2af large subunit B"
FT                   /id="PRO_0000352270"
FT   DOMAIN          239..322
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          359..437
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          478..564
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..187
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        12..28
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..142
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        143..170
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   573 AA;  63866 MW;  28A81B4481ACC2B5 CRC64;
     MPDYEGNGED IDNYTGGSSP PPKSRSSHGH GPTPDDYSDS KSQHSSRENE KDRDSSRSRE
     KDRERGRDKD RDRDRDRDRG RDRDRGRDRD KDREREKDRD RHHGDRHRDR SDRREKERTR
     DRDDDDRHRT RDYDQQREHA KDRESRHRHR SRSRGRSEHR SRSRSRSRSK SKRISGFDMA
     PPTSAMLPGI TAAAGQVPGT NPPIPGMFPN MFPLASGQFG ALPVMPIQAM TQQATRHARR
     VYVGGLPAHA NEQSVATFFS HVMSAIGGNT AGPGDAVVNV YINYEKKFAF VEMRSVEEAS
     NAMALDGIIF EGAPCKVRRP SDYNPSLAAT LGPSQPNPNL NLAAVGLSPG SAGGLEGPDR
     IFVGGLPYYF TEAQIRELLE SFGPLRGFDL VKDRETGNSK GYAFCVYQDV SVTDIACAAL
     NGIKMGDKTL TVRRANQGTT QPKPEQESVL LHAQQQIALQ RLMLQPATLA TKVLSLTEVI
     SADELNDDED YQDILEDMRT ECGKFGSLVN VVIPRPSPNG EPTPGVGKVF LEYADVDSSS
     KARQSLNGRK FGGNQVVAVF YPENKFYEGD YDG