U2A2B_ORYSJ
ID U2A2B_ORYSJ Reviewed; 548 AA.
AC Q2QZL4; Q0IR43;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Splicing factor U2af large subunit B;
DE AltName: Full=U2 auxiliary factor 65 kDa subunit B;
DE AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit B;
DE Short=U2 snRNP auxiliary factor large subunit B;
GN Name=U2AF65B; OrderedLocusNames=Os11g0682300, LOC_Os11g45590;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Necessary for the splicing of pre-mRNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: N-terminal RS domain has a very strong bias in favor of D over
CC S.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF28822.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DP000010; ABA95281.2; -; Genomic_DNA.
DR EMBL; AP008217; BAF28822.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014967; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015617533.1; XM_015762047.1.
DR AlphaFoldDB; Q2QZL4; -.
DR SMR; Q2QZL4; -.
DR STRING; 4530.OS11T0682300-01; -.
DR PaxDb; Q2QZL4; -.
DR PRIDE; Q2QZL4; -.
DR GeneID; 4351124; -.
DR KEGG; osa:4351124; -.
DR eggNOG; KOG0120; Eukaryota.
DR InParanoid; Q2QZL4; -.
DR OrthoDB; 896650at2759; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR InterPro; IPR006529; U2AF_lg.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 3.
DR SMART; SM00361; RRM_1; 1.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR PROSITE; PS50102; RRM; 2.
PE 3: Inferred from homology;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..548
FT /note="Splicing factor U2af large subunit B"
FT /id="PRO_0000352272"
FT DOMAIN 214..297
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 334..412
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 453..539
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..117
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..146
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 548 AA; 61360 MW; 18B0194DA6E768B8 CRC64;
MADDHAAAAD LVDGGRPEGD THSREDGLSK PRDKDREREK DKDRERHRDR DRDRGRDRDR
DKDKEKDRDK ERDRDRDRDK DRDRHHRHHR ERREHRDRSD DHDRHRSRDS ERRRDHERDG
RRRHRSRSRS RSRGRDRRSR SRSRSKSKRV SGFDMAPPAQ AVVPQFPAIP TPSQFPGTAI
PGMFPNMLPM GVGQFNPLVI QPQAMTQQAT RHARRVYVGG LPPTANEQSV AIYFNQVMAA
IGGNTAGPGD AVLNVYINHD KKFAFVEMRS VEEASNAMAL DGILFEGAPV KVRRPTDYNP
SLAAALGPSQ PSPNLNLAAV GLTPGSAGGL EGPDRIFVGG LPYYFTEAQV RELLESFGPL
RGFDLVKDRE TGNSKGYAFC VYQDLNVTDI ACAALNGIKM GDKTLTVRRA NQGAAQPRPE
QESILLQAQQ QVQLQKLVYQ VGALPTKVVC LTQVVSADEL KDDEEYEDIM EDMRLEAGKY
GNLIKVVIPR PDPSGLPVAG VGKVFLEYAD VDGATKAKTA MHGRKFGGNP VVAVFYPENK
FSSAEYDA
