ID   U2A2B_WHEAT             Reviewed;         543 AA.
AC   Q2QKB4;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Splicing factor U2af large subunit B;
DE   AltName: Full=U2 auxiliary factor 65 kDa subunit B;
DE   AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit B;
DE            Short=U2 snRNP auxiliary factor large subunit B;
GN   Name=U2AF65B; Synonyms=U2AF65;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Chinese Spring; TISSUE=Grain;
RX   PubMed=16941218; DOI=10.1007/s11103-006-9046-6;
RA   Lopato S., Borisjuk L., Milligan A.S., Shirley N., Bazanova N.,
RA   Langridge P.;
RT   "Systematic identification of factors involved in post-transcriptional
RT   processes in wheat grain.";
RL   Plant Mol. Biol. 62:637-653(2006).
CC   -!- FUNCTION: Necessary for the splicing of pre-mRNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: N-terminal RS domain has a very strong bias in favor of D over
CC       S.
CC   -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR   EMBL; DQ019635; AAY84880.1; -; mRNA.
DR   AlphaFoldDB; Q2QKB4; -.
DR   SMR; Q2QKB4; -.
DR   STRING; 4565.Traes_4DS_FABA6C874.2; -.
DR   PRIDE; Q2QKB4; -.
DR   EnsemblPlants; TraesPAR_scaffold_060662_01G000300.1; TraesPAR_scaffold_060662_01G000300.1; TraesPAR_scaffold_060662_01G000300.
DR   EnsemblPlants; TraesWEE_scaffold_052746_01G000300.1; TraesWEE_scaffold_052746_01G000300.1; TraesWEE_scaffold_052746_01G000300.
DR   Gramene; TraesPAR_scaffold_060662_01G000300.1; TraesPAR_scaffold_060662_01G000300.1; TraesPAR_scaffold_060662_01G000300.
DR   Gramene; TraesWEE_scaffold_052746_01G000300.1; TraesWEE_scaffold_052746_01G000300.1; TraesWEE_scaffold_052746_01G000300.
DR   eggNOG; KOG0120; Eukaryota.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; Q2QKB4; baseline and differential.
DR   GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR   GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR   GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR   GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR   GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR   GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.330; -; 3.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR006529; U2AF_lg.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 3.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW   RNA-binding.
FT   CHAIN           1..543
FT                   /note="Splicing factor U2af large subunit B"
FT                   /id="PRO_0000352274"
FT   DOMAIN          207..290
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          327..405
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          446..532
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..171
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..78
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        87..111
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        112..126
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        154..171
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   543 AA;  60586 MW;  4407A0245178FFFE CRC64;
     MADDNGGGGD DYVSEAVRPE GDTHTREEGL SKSRDRDREK DKDKERHRDR DRDRGRDRDR
     GRDRDLDKDR DRDKDRDRHQ RHHRDKREHR DRPDDHDRHR SRDSERRRDR ERDGHRRHRS
     RSRSRSRGRD DHRSRSHSKS KRVSGFDLGP TAQSVLPQFP TIPTPSQLPG SSIPGMFPNM
     LPFADGQINP LVMQPQAMTQ QATRHARRVY VGGLPPSANE QSVAIYFNQV MAAIGGNTAG
     PGDAVLNVYI NHDKKFAFVE MRSVEEASNA MALDGILFEG APVKVRRPTD YNPSLAAALG
     PSQPSSNLNL AAVGLTPGSA GGLEGPDRIF VGGLPYYFTE AQVRELLESF GPLRGFDLVK
     DRETGNSKGY AFCVYQDLNV TDIACAALNG IKMGDKTLTV RRANQGSAQP RPEQENILLQ
     AQQQVQLQKL VYQVGALPTK VVCLTQVVTA DELKDDEEYE DIMEDMRLEA GKYGNLVKVV
     IPRPHPSGEP VSGVGKVFLE YADVDGSTKA KTAMHGRKFG GNPVVAVFYP ENKFADEDYD
     AAA