U2AF1_MOUSE
ID U2AF1_MOUSE Reviewed; 239 AA.
AC Q9D883; Q564E4; Q99LX2; Q9CZ98;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Splicing factor U2AF 35 kDa subunit;
DE AltName: Full=U2 auxiliary factor 35 kDa subunit;
DE AltName: Full=U2 snRNP auxiliary factor small subunit;
GN Name=U2af1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone marrow, Small intestine, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PHF5A, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18758164; DOI=10.1159/000138890;
RA Rzymski T., Grzmil P., Meinhardt A., Wolf S., Burfeind P.;
RT "PHF5A represents a bridge protein between splicing proteins and ATP-
RT dependent helicases and is differentially expressed during mouse
RT spermatogenesis.";
RL Cytogenet. Genome Res. 121:232-244(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a critical role in both constitutive and enhancer-
CC dependent splicing by mediating protein-protein interactions and
CC protein-RNA interactions required for accurate 3'-splice site
CC selection. Recruits U2 snRNP to the branch point. Directly mediates
CC interactions between U2AF2 and proteins bound to the enhancers and thus
CC may function as a bridge between U2AF2 and the enhancer complex to
CC recruit it to the adjacent intron (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Heterodimer with
CC U2AF2. Interacts with ZRANB2 (By similarity). Interacts (via RS domain)
CC with PHF5A (via N-terminus). {ECO:0000250,
CC ECO:0000269|PubMed:18758164}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18758164}. Nucleus
CC speckle {ECO:0000269|PubMed:18758164}.
CC -!- TISSUE SPECIFICITY: Expressed in primary spermatocytes and elongating
CC spermatids (at protein level). {ECO:0000269|PubMed:18758164}.
CC -!- DOMAIN: The C-terminal SR-rich domain is required for interactions with
CC SR proteins and the splicing regulators TRA and TRA2, and the N-
CC terminal domain is required for formation of the U2AF1/U2AF2
CC heterodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR EMBL; AK008332; BAB25609.1; -; mRNA.
DR EMBL; AK012849; BAB28511.1; -; mRNA.
DR EMBL; AK088979; BAC40683.1; -; mRNA.
DR EMBL; AK150872; BAE29923.1; -; mRNA.
DR EMBL; BC002184; AAH02184.1; -; mRNA.
DR CCDS; CCDS28611.1; -.
DR RefSeq; NP_001157241.1; NM_001163769.1.
DR RefSeq; NP_077149.2; NM_024187.4.
DR AlphaFoldDB; Q9D883; -.
DR SMR; Q9D883; -.
DR BioGRID; 223844; 8.
DR IntAct; Q9D883; 3.
DR MINT; Q9D883; -.
DR STRING; 10090.ENSMUSP00000014684; -.
DR iPTMnet; Q9D883; -.
DR PhosphoSitePlus; Q9D883; -.
DR SwissPalm; Q9D883; -.
DR EPD; Q9D883; -.
DR jPOST; Q9D883; -.
DR MaxQB; Q9D883; -.
DR PaxDb; Q9D883; -.
DR PRIDE; Q9D883; -.
DR ProteomicsDB; 298083; -.
DR DNASU; 108121; -.
DR Ensembl; ENSMUST00000014684; ENSMUSP00000014684; ENSMUSG00000061613.
DR GeneID; 108121; -.
DR KEGG; mmu:108121; -.
DR UCSC; uc008bvo.2; mouse.
DR CTD; 7307; -.
DR MGI; MGI:98884; U2af1.
DR VEuPathDB; HostDB:ENSMUSG00000061613; -.
DR eggNOG; KOG2202; Eukaryota.
DR GeneTree; ENSGT00950000183152; -.
DR InParanoid; Q9D883; -.
DR OMA; NDGEDRW; -.
DR OrthoDB; 1340384at2759; -.
DR PhylomeDB; Q9D883; -.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72187; mRNA 3'-end processing.
DR Reactome; R-MMU-73856; RNA Polymerase II Transcription Termination.
DR BioGRID-ORCS; 108121; 25 hits in 72 CRISPR screens.
DR ChiTaRS; U2af1; mouse.
DR PRO; PR:Q9D883; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9D883; protein.
DR Bgee; ENSMUSG00000061613; Expressed in epiblast (generic) and 112 other tissues.
DR ExpressionAtlas; Q9D883; baseline and differential.
DR Genevisible; Q9D883; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005681; C:spliceosomal complex; ISO:MGI.
DR GO; GO:0089701; C:U2AF complex; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR GO; GO:0050733; F:RS domain binding; IPI:MGI.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR InterPro; IPR009145; U2AF_small.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR12620; PTHR12620; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF00642; zf-CCCH; 2.
DR PRINTS; PR01848; U2AUXFACTOR.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00361; RRM_1; 1.
DR SMART; SM00356; ZnF_C3H1; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS50103; ZF_C3H1; 2.
PE 1: Evidence at protein level;
KW Acetylation; Metal-binding; Methylation; mRNA processing; mRNA splicing;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding;
KW Spliceosome; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT CHAIN 2..239
FT /note="Splicing factor U2AF 35 kDa subunit"
FT /id="PRO_0000081995"
FT DOMAIN 65..147
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT ZN_FING 12..40
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 149..176
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 183..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..205
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT MOD_RES 39
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT MOD_RES 165
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q01081"
FT CONFLICT 187
FT /note="G -> R (in Ref. 1; BAB25609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 27815 MW; DFF944210581244D CRC64;
MAEYLASIFG TEKDKVNCSF YFKIGACRHG DRCSRLHNKP TFSQTIALLN IYRNPQNSSQ
SADGLRCAVS DVEMQEHYDE FFEEVFTEME EKYGEVEEMN VCDNLGDHLV GNVYVKFRRE
EDAEKAVIDL NNRWFNGQPI HAELSPVTDF REACCRQYEM GECTRGGFCN FMHLKPISRE
LRRELYGRRR KKHRSRSRSR ERRSRSRDRG RGGGGGGGGG GGRERDRRRS RDRERSGRF
