U2AF2_CAEBR
ID U2AF2_CAEBR Reviewed; 488 AA.
AC P90727; A8XLT8; Q616T0;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Splicing factor U2AF 65 kDa subunit;
DE AltName: Full=U2 auxiliary factor 65 kDa subunit;
DE Short=U2AF65;
DE AltName: Full=U2 snRNP auxiliary factor large subunit;
GN Name=uaf-1; ORFNames=CBG15152;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9001248; DOI=10.1128/mcb.17.2.946;
RA Zorio D.A.R., Lea K., Blumenthal T.;
RT "Cloning of Caenorhabditis U2AF65: an alternatively spliced RNA containing
RT a novel exon.";
RL Mol. Cell. Biol. 17:946-953(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Necessary for the splicing of pre-mRNA. Binds to the
CC polypyrimidine tract of introns early during spliceosome assembly (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a heterodimer with the U2AF small subunit.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U79145; AAB38280.1; -; Genomic_DNA.
DR EMBL; HE601055; CAP33592.1; -; Genomic_DNA.
DR RefSeq; XP_002642882.1; XM_002642836.1.
DR AlphaFoldDB; P90727; -.
DR SMR; P90727; -.
DR STRING; 6238.CBG15152; -.
DR PRIDE; P90727; -.
DR EnsemblMetazoa; CBG15152a.1; CBG15152a.1; WBGene00035480.
DR GeneID; 8584875; -.
DR KEGG; cbr:CBG_15152; -.
DR CTD; 8584875; -.
DR WormBase; CBG15152a; CBP18334; WBGene00035480; Cbr-uaf-1.
DR eggNOG; KOG0120; Eukaryota.
DR HOGENOM; CLU_021795_4_2_1; -.
DR InParanoid; P90727; -.
DR OMA; MTQWDIK; -.
DR OrthoDB; 896650at2759; -.
DR Proteomes; UP000008549; Chromosome III.
DR GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR GO; GO:0048589; P:developmental growth; IEA:EnsemblMetazoa.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IEA:EnsemblMetazoa.
DR GO; GO:0010172; P:embryonic body morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007281; P:germ cell development; IEA:EnsemblMetazoa.
DR GO; GO:0000389; P:mRNA 3'-splice site recognition; IEA:EnsemblMetazoa.
DR GO; GO:0002119; P:nematode larval development; IEA:EnsemblMetazoa.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR006529; U2AF_lg.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR PROSITE; PS50102; RRM; 3.
PE 3: Inferred from homology;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..488
FT /note="Splicing factor U2AF 65 kDa subunit"
FT /id="PRO_0000081990"
FT DOMAIN 175..257
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 282..359
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 389..479
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 25..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..129
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 5
FT /note="A -> S (in Ref. 1; AAB38280)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 488 AA; 55628 MW; 784BE4878592752A CRC64;
MVEQAQGSLE EIERQFLDVA QRDGLESLQE DVKPDVKSDL NGNGEEKRDR DDEDRKKRKR
SRSRDRGDRD RKRSRSRDRR DRDRSRSRER RRDRSRDRNR DDRRGGRDDD RRREPQEPAK
PREPKKYRFW DVPPTGFENI TPMEYKNMQA SGAVPRGSVQ SAVPVVGPSV TCQSRRLYVG
NIPFGCNEEA MLDFFNQQMH LCNLAQAPGN PILLCQINLD KNFAFIEFRS IDETTAGMAF
DGINFMGQQL KVRRPRDYQP SQNTFDMNAR MPVSSIVVDS ANKIFIGGLP NYLTEDQVKE
LLCSFGPLKA FSLNVDSQGN SKGYAFAEYL DPTLTDQAIA GLNGMQLGDK QLVVQLACAN
QTRHNTHLPN SASAIAGIDL SQGAGRATEI LCLMNMVTED ELRSDEDYEE ILEDVREECS
KYGIVRSLEI PRPYDDHPVP GVGKVFVEFA TTSDCQRAQA ALTGRKFANR TVVTSYYDVD
KYHNRQFN
