U2AF2_CAEEL
ID U2AF2_CAEEL Reviewed; 496 AA.
AC P90978; O76839; Q95XT0; Q9N2X5;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Splicing factor U2AF 65 kDa subunit;
DE AltName: Full=U2 auxiliary factor 65 kDa subunit;
DE Short=U2AF65;
DE AltName: Full=U2 snRNP auxiliary factor large subunit;
GN Name=uaf-1; ORFNames=Y92C3B.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC STRAIN=Bristol N2; TISSUE=Embryo;
RX PubMed=9001248; DOI=10.1128/mcb.17.2.946;
RA Zorio D.A.R., Lea K., Blumenthal T.;
RT "Cloning of Caenorhabditis U2AF65: an alternatively spliced RNA containing
RT a novel exon.";
RL Mol. Cell. Biol. 17:946-953(1997).
RN [2]
RP SEQUENCE REVISION.
RA Zorio D.A.R.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Necessary for the splicing of pre-mRNA. Binds to the
CC polypyrimidine tract of introns early during spliceosome assembly (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a heterodimer with the U2AF small subunit.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Experimental confirmation may be lacking for some isoforms.;
CC Name=A;
CC IsoId=P90978-1; Sequence=Displayed;
CC Name=B;
CC IsoId=P90978-2; Sequence=VSP_005904;
CC Name=C;
CC IsoId=P90978-3; Sequence=VSP_005905;
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DR EMBL; U79157; AAC26982.1; -; mRNA.
DR EMBL; FO080242; CCD62278.1; -; Genomic_DNA.
DR EMBL; FO080242; CCD62279.1; -; Genomic_DNA.
DR EMBL; FO080242; CCD62280.1; -; Genomic_DNA.
DR RefSeq; NP_001022967.1; NM_001027796.3. [P90978-1]
DR RefSeq; NP_001022969.1; NM_001027798.1.
DR RefSeq; NP_497326.2; NM_064925.6.
DR AlphaFoldDB; P90978; -.
DR SMR; P90978; -.
DR BioGRID; 40534; 15.
DR IntAct; P90978; 7.
DR STRING; 6239.Y92C3B.2a; -.
DR iPTMnet; P90978; -.
DR EPD; P90978; -.
DR PaxDb; P90978; -.
DR PeptideAtlas; P90978; -.
DR EnsemblMetazoa; Y92C3B.2a.1; Y92C3B.2a.1; WBGene00006697. [P90978-1]
DR EnsemblMetazoa; Y92C3B.2b.1; Y92C3B.2b.1; WBGene00006697. [P90978-2]
DR GeneID; 175270; -.
DR KEGG; cel:CELE_Y92C3B.2; -.
DR UCSC; Y92C3B.2b.1; c. elegans. [P90978-1]
DR CTD; 175270; -.
DR WormBase; Y92C3B.2a; CE27339; WBGene00006697; uaf-1. [P90978-1]
DR WormBase; Y92C3B.2b; CE25626; WBGene00006697; uaf-1. [P90978-2]
DR WormBase; Y92C3B.2c; CE29149; WBGene00006697; uaf-1. [P90978-3]
DR eggNOG; KOG0120; Eukaryota.
DR GeneTree; ENSGT00940000155556; -.
DR InParanoid; P90978; -.
DR OMA; MTQWDIK; -.
DR OrthoDB; 896650at2759; -.
DR PhylomeDB; P90978; -.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72187; mRNA 3'-end processing.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR PRO; PR:P90978; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00006697; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase.
DR GO; GO:0005681; C:spliceosomal complex; ISS:WormBase.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IDA:WormBase.
DR GO; GO:0048589; P:developmental growth; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0010172; P:embryonic body morphogenesis; IMP:WormBase.
DR GO; GO:0007281; P:germ cell development; IMP:WormBase.
DR GO; GO:0000389; P:mRNA 3'-splice site recognition; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; ISS:WormBase.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR006529; U2AF_lg.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01642; U2AF_lg; 1.
DR PROSITE; PS50102; RRM; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..496
FT /note="Splicing factor U2AF 65 kDa subunit"
FT /id="PRO_0000081991"
FT DOMAIN 184..266
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 291..368
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 404..488
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..353
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_005904"
FT VAR_SEQ 57..78
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_005905"
SQ SEQUENCE 496 AA; 55431 MW; 1A967DE89DC9373C CRC64;
MSDHQDGMKL EDIERQFLDV AQREGGLEAI QPTTGPLENE ENLKSSTGGG GGEDDNDRKK
RKRSRSRDRD TRRRSRSRDR GERRGGGGGG DRDRSRSRER RRGGGGRDEP RRRGGDDEAR
SRREPEPQKP REPKKYRFWD VPPTGFETTT PMEYKNMQAA GQVPRGSVQS AVPVVGPSVT
CQSRRLYVGN IPFGCNEEAM LDFFNQQMHL CGLAQAPGNP ILLCQINLDK NFAFIEFRSI
DETTAGMAFD GINFMGQQLK VRRPRDYQPS QNTFDMNSRM PVSTIVVDSA NKIFIGGLPN
YLTEDQVKEL LCSFGPLKAF SLNVDSQGNS KGYAFAEYLD PTLTDQAIAG LNGMQLGDKQ
LVVQLACANQ QRHNTNLPNS ASAIAGIDLS QGAGRATEIL CLMNMVTEDE LKADDEYEEI
LEDVRDECSK YGIVRSLEIP RPYEDHPVPG VGKVFVEFAS TSDCQRAQAA LTGRKFANRT
VVTSYYDVDK YHNRQF
