C74A1_ORYSJ
ID C74A1_ORYSJ Reviewed; 512 AA.
AC Q7Y0C8;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Allene oxide synthase 1, chloroplastic;
DE EC=4.2.1.92;
DE AltName: Full=Cytochrome P450 74A1;
DE AltName: Full=Hydroperoxide dehydrase 1;
DE Flags: Precursor;
GN Name=CYP74A1; Synonyms=AOS1;
GN OrderedLocusNames=Os03g0767000, LOC_Os03g55800;
GN ORFNames=OSJNBa0079B15.7, OSJNBb0106M04.24;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, AND
RP NOMENCLATURE.
RC STRAIN=cv. Nipponbare; TISSUE=Seedling leaf;
RX PubMed=14988482; DOI=10.1093/pcp/pch025;
RA Haga K., Iino M.;
RT "Phytochrome-mediated transcriptional up-regulation of ALLENE OXIDE
RT SYNTHASE in rice seedlings.";
RL Plant Cell Physiol. 45:119-128(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in the biosynthesis of jasmonic acid, a growth
CC regulator that is implicated also as a signaling molecule in plant
CC defense. Converts 13-hydroperoxylinolenic acid to 12,13-epoxylinolenic
CC acid (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(13S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoate =
CC (9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O;
CC Xref=Rhea:RHEA:25074, ChEBI:CHEBI:15377, ChEBI:CHEBI:36438,
CC ChEBI:CHEBI:58757; EC=4.2.1.92;
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in coleoptiles, and at lower level in
CC leaves of dark-grown seedlings. {ECO:0000269|PubMed:14988482}.
CC -!- INDUCTION: Transiently induced by red (R) light, far-red (FR) light and
CC abrasion in dark-grown seedlings. Not induced by FR light in
CC phytochrome A (phyA) mutant. {ECO:0000269|PubMed:14988482}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AB116527; BAD08330.1; -; mRNA.
DR EMBL; AC099043; AAP50956.1; -; Genomic_DNA.
DR EMBL; AC107207; AAR87328.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86563.1; -; Genomic_DNA.
DR RefSeq; XP_015631686.1; XM_015776200.1.
DR AlphaFoldDB; Q7Y0C8; -.
DR SMR; Q7Y0C8; -.
DR STRING; 4530.OS03T0767000-01; -.
DR PaxDb; Q7Y0C8; -.
DR PRIDE; Q7Y0C8; -.
DR EnsemblPlants; Os03t0767000-01; Os03t0767000-01; Os03g0767000.
DR GeneID; 4334233; -.
DR Gramene; Os03t0767000-01; Os03t0767000-01; Os03g0767000.
DR KEGG; osa:4334233; -.
DR eggNOG; ENOG502QQNS; Eukaryota.
DR HOGENOM; CLU_045757_0_0_1; -.
DR InParanoid; Q7Y0C8; -.
DR OMA; KVTMAAM; -.
DR OrthoDB; 485250at2759; -.
DR PlantReactome; R-OSA-1119332; Jasmonic acid biosynthesis.
DR UniPathway; UPA00382; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q7Y0C8; baseline and differential.
DR Genevisible; Q7Y0C8; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009978; F:allene oxide synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:EnsemblPlants.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009695; P:jasmonic acid biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009753; P:response to jasmonic acid; IEA:EnsemblPlants.
DR GO; GO:0009611; P:response to wounding; IEA:EnsemblPlants.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism; Heme; Iron;
KW Lipid biosynthesis; Lipid metabolism; Lyase; Membrane; Metal-binding;
KW Oxylipin biosynthesis; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..25
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 26..512
FT /note="Allene oxide synthase 1, chloroplastic"
FT /id="PRO_0000003627"
FT REGION 23..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 131
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 314..315
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 321
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 381..384
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 465
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 512 AA; 56504 MW; 27B86DCE56157AAF CRC64;
MATAAACISF ASPSPARVVI RRQTRASASA SATDRQEVVS PKRRLPLRKV PGDYGPPVVG
AIRDRYEYFY GPGGRDGFFA ARVRAHRSTV VRLNMPPGPF VARDPRVVAL LDAASFPVLF
DTSLVDKTDL FTGTFMPSTD LTGGYRVLSY LDPSEPNHAP LKTLLFYLLS HRRQQVIPKF
REVYGDLFGL MENDLARVGK ADFGVHNDAA AFGFLCQGLL GRDPAKSALG RDGPKLITKW
VLFQLSPLLS LGLPTLVEDT LLHSLRLPPA LVKKDYDRLA DFFRDAAKAV VDEGERLGIA
REEAVHNILF ALCFNSFGGM KILFPTLVKW LGRAGARVHG RLATEVRGAV RDNGGEVTMK
ALAEMPLVKS AVYEALRIEP PVAMQYGRAK RDMVVESHDY GYEVREGEML FGYQPMATKD
PRVFARPEEY VPDRFLGEDG ARLLRHVVWS NGPETAAPTL HDKQCAGKDF VVLVARLLLV
ELFLRYDSFD VEVGTSTLGS SVTVTSLKKA TF
