U2AFL_HUMAN
ID U2AFL_HUMAN Reviewed; 479 AA.
AC Q15695; B2R901; Q13570; Q2M3R8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Putative U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 1 {ECO:0000305};
DE AltName: Full=CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 1;
DE AltName: Full=U2(RNU2) small nuclear RNA auxiliary factor 1-like 1;
GN Name=ZRSR2P1 {ECO:0000312|HGNC:HGNC:12456};
GN Synonyms=U2AF1-RS1, U2AF1L1, U2AF1P, U2AF1RS1, U2AFBPL, ZRSR1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=8586425; DOI=10.1006/geno.1995.9879;
RA Kitagawa K., Wang X., Hatada I., Yamaoka T., Nojima H., Inazawa J., Abe T.,
RA Mitsuya K., Oshimura M., Murata A., Monden M., Mukai T.;
RT "Isolation and mapping of human homologues of an imprinted mouse gene
RT U2af1-rs1.";
RL Genomics 30:257-263(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8630064; DOI=10.1006/bbrc.1996.0716;
RA Pearsall R.S., Shibata H., Brozowska A., Yoshino K., Okuda K., Dejong P.J.,
RA Plass C., Chapman V.M., Hayashizaki Y., Held W.A.;
RT "Absence of imprinting in U2AFBPL, a human homologue of the imprinted mouse
RT gene U2afbp-rs.";
RL Biochem. Biophys. Res. Commun. 222:171-177(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Small intestine;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
CC -!- INTERACTION:
CC Q15695; O60333-2: KIF1B; NbExp=3; IntAct=EBI-12270264, EBI-10975473;
CC Q15695; P55081: MFAP1; NbExp=3; IntAct=EBI-12270264, EBI-1048159;
CC Q15695; Q6BDI9: REP15; NbExp=5; IntAct=EBI-12270264, EBI-12048237;
CC Q15695; A7MD48: SRRM4; NbExp=3; IntAct=EBI-12270264, EBI-3867173;
CC Q15695; P26368-2: U2AF2; NbExp=3; IntAct=EBI-12270264, EBI-11097439;
CC Q15695; Q15696: ZRSR2; NbExp=4; IntAct=EBI-12270264, EBI-6657923;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. Appears to have arisen
CC by retrotransposition of ZRSR2. Its uncertain if ZRSR2P1 is the
CC ortholog of mouse ZRSR1, synteny is not conserved. {ECO:0000305}.
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DR EMBL; D49676; BAA08532.1; -; mRNA.
DR EMBL; U51224; AAA98669.1; -; Genomic_DNA.
DR EMBL; AK313576; BAG36348.1; -; mRNA.
DR EMBL; AC008536; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q15695; -.
DR SMR; Q15695; -.
DR IntAct; Q15695; 9.
DR STRING; 9606.ENSP00000375133; -.
DR iPTMnet; Q15695; -.
DR PhosphoSitePlus; Q15695; -.
DR SwissPalm; Q15695; -.
DR BioMuta; ZRSR1; -.
DR DMDM; 2833265; -.
DR jPOST; Q15695; -.
DR MassIVE; Q15695; -.
DR MaxQB; Q15695; -.
DR PaxDb; Q15695; -.
DR PeptideAtlas; Q15695; -.
DR PRIDE; Q15695; -.
DR ProteomicsDB; 60703; -.
DR UCSC; uc021ycm.2; human.
DR GeneCards; ZRSR2P1; -.
DR HGNC; HGNC:12456; ZRSR2P1.
DR MIM; 601079; gene.
DR neXtProt; NX_Q15695; -.
DR eggNOG; KOG2202; Eukaryota.
DR HOGENOM; CLU_029117_1_0_1; -.
DR InParanoid; Q15695; -.
DR PhylomeDB; Q15695; -.
DR TreeFam; TF324447; -.
DR PathwayCommons; Q15695; -.
DR Pharos; Q15695; Tdark.
DR PRO; PR:Q15695; -.
DR Proteomes; UP000005640; Unplaced.
DR RNAct; Q15695; protein.
DR Genevisible; Q15695; HS.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR InterPro; IPR009145; U2AF_small.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR12620; PTHR12620; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF00642; zf-CCCH; 1.
DR PRINTS; PR01848; U2AUXFACTOR.
DR SMART; SM00361; RRM_1; 1.
DR SMART; SM00356; ZnF_C3H1; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS50103; ZF_C3H1; 2.
PE 5: Uncertain;
KW Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Ribonucleoprotein; RNA-binding;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..479
FT /note="Putative U2 small nuclear ribonucleoprotein
FT auxiliary factor 35 kDa subunit-related protein 1"
FT /id="PRO_0000081999"
FT DOMAIN 203..309
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT ZN_FING 171..199
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 311..338
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 356..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 363..405
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..429
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..466
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 354
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q15696"
FT MOD_RES 389
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q15696"
FT CROSSLNK 67
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q15696"
FT CONFLICT 135
FT /note="K -> E (in Ref. 3; BAG36348)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="K -> R (in Ref. 3; BAG36348)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 479 AA; 57643 MW; 96F326694BD4E7C0 CRC64;
MAALEKMTFP KKMTFPEKPS HKKYRAALKK EKRKKRRQEL ARLRDSGLSQ EEEEDTFIEE
QQLEEEKLLE RERERLHEEW LLREQKAQEE FRIKKEKEEA AKKWLEEQER KLKEQWKEQQ
RKEREEEEQK QQEKKEKEEA VQKMLDQAEN DLENSTTWQN PEPPVDFRVM EKDRANCPFY
SKTGACRFGD RCSRKHNFPT SSPTLLIKSM FTTFGMEQCR RDDYDPDASL EYSEEETYQQ
FLDFYEDVLP EFKNVGKVIQ FKVSCNLEPH LRGNVYVQYQ SEEECQAALS LFNGRWYAGR
QLQCEFCPVT RWKMAICGLF EIQQCPRGKH CNFLHVFRNP NNEFWEANRD IYLSSDQTGS
SFGKNSERRE KMGHHDHYYS RQRGRRNPSP DHTYKRNGES ERKKSSHRGK KSHKRTSKSR
ERHNSPSRGR NRHRSWDQGR RSQSRRSHRS RSQSSSRCRS RGRRKSGNRD RTVQSPQSK
