U2D2B_RAT
ID U2D2B_RAT Reviewed; 147 AA.
AC P70711;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 D2B;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme D2B;
DE AltName: Full=Ubiquitin carrier protein D2B;
DE AltName: Full=Ubiquitin-conjugating enzyme E2(17)KB 2B;
DE AltName: Full=Ubiquitin-conjugating enzyme E2-17 kDa 2B;
DE AltName: Full=Ubiquitin-protein ligase D2B;
GN Name=Ube2d2b; Synonyms=Ube2d2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=8754804; DOI=10.1128/mcb.16.8.4064;
RA Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.;
RT "A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating
RT enzymes UBC4 and UBC5 with distinct biochemical features is induced during
RT spermatogenesis.";
RL Mol. Cell. Biol. 16:4064-4072(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Mediates the selective degradation of short-lived and
CC abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of
CC p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of
CC STUB1 and TRAF6. Involved in the signal-induced conjugation and
CC subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination
CC and degradation, MDM2-dependent degradation of p53/TP53 and the
CC activation of MAVS in the mitochondria by DDX58/RIG-I in response to
CC viral infection Plays a role in early maturation of the testis.
CC {ECO:0000250|UniProtKB:P62837, ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P62837, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with CNOT4 (via RING domain).
CC {ECO:0000250|UniProtKB:P62837}.
CC -!- TISSUE SPECIFICITY: Testis-specific. Mainly expressed in the round
CC spermatids (at protein level). {ECO:0000269|PubMed:8754804}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; U56407; AAC52942.1; -; mRNA.
DR EMBL; BC078808; AAH78808.1; -; mRNA.
DR RefSeq; NP_112263.1; NM_031001.2.
DR AlphaFoldDB; P70711; -.
DR SMR; P70711; -.
DR STRING; 10116.ENSRNOP00000039621; -.
DR iPTMnet; P70711; -.
DR PhosphoSitePlus; P70711; -.
DR PaxDb; P70711; -.
DR GeneID; 79435; -.
DR KEGG; rno:79435; -.
DR UCSC; RGD:69425; rat.
DR CTD; 73318; -.
DR RGD; 69425; RGD69425.
DR eggNOG; KOG0417; Eukaryota.
DR HOGENOM; CLU_030988_13_3_1; -.
DR InParanoid; P70711; -.
DR OMA; ISEANMY; -.
DR OrthoDB; 1337945at2759; -.
DR PhylomeDB; P70711; -.
DR TreeFam; TF101108; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:P70711; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000034016; Expressed in testis and 2 other tissues.
DR Genevisible; P70711; RN.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; ISO:RGD.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISO:RGD.
DR GO; GO:1903841; P:cellular response to arsenite(3-); IEP:RGD.
DR GO; GO:0071276; P:cellular response to cadmium ion; IEP:RGD.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISO:RGD.
DR GO; GO:0044314; P:protein K27-linked ubiquitination; ISO:RGD.
DR GO; GO:0035519; P:protein K29-linked ubiquitination; ISO:RGD.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISO:RGD.
DR GO; GO:0085020; P:protein K6-linked ubiquitination; ISO:RGD.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISO:RGD.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; ISO:RGD.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..147
FT /note="Ubiquitin-conjugating enzyme E2 D2B"
FT /id="PRO_0000082469"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 147 AA; 16659 MW; E950720AEC2AB2B2 CRC64;
MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY
PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV
PEIAQIYKTD RDKYNRTARE WTQKYAM
