ID   U321_DORVU              Reviewed;          56 AA.
AC   P0DUS7;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 4.
DE   RecName: Full=U-limacoditoxin(3)-Dv21 {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(3)-Dv21 {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Cecropin-like peptide {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-56, FUNCTION,
RP   SUBCELLULAR LOCATION, RECOMBINANT EXPRESSION, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Probable toxin. Shows a moderate antiparasitic activity
CC       against the major pathogenic nematode of ruminants (H.contortus,
CC       IC(50)=22.1 uM). Does not show insecticidal activities. Does not induce
CC       increase in intracellular calcium in mouse DRG neurons, suggesting that
CC       it does not induce pain. {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000305|PubMed:33893140}.
CC   -!- SIMILARITY: Belongs to the limacoditoxin-22 family. {ECO:0000305}.
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DR   SMR; P0DUS7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019855; F:calcium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012325; Assassin_bug_toxin-like.
DR   Pfam; PF08117; Toxin_30; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         20..56
FT                   /note="U-limacoditoxin(3)-Dv21"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453403"
FT   DISULFID        26..41
FT                   /evidence="ECO:0000305|PubMed:33893140"
FT   DISULFID        33..46
FT                   /evidence="ECO:0000305|PubMed:33893140"
FT   DISULFID        40..53
FT                   /evidence="ECO:0000305|PubMed:33893140"
SQ   SEQUENCE   56 AA;  6113 MW;  5F8A7D22EA20CF9E CRC64;
     MKKVIMLLLI FALFAYALSA SDPNKCLKKG SKCVSVGKPC CKPATCNIYA NRCIGW