ID   U332_DORVU              Reviewed;          59 AA.
AC   P0DUS8;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=U-limacoditoxin(3)-Dv32a {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(3)-Dv32a {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-59, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Probable toxin. Does not induce increase in intracellular
CC       calcium in mouse DRG neurons, suggesting that it does not induce pain.
CC       {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000305|PubMed:33893140}.
CC   -!- MASS SPECTROMETRY: Mass=4806.0; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:33893140};
CC   -!- SIMILARITY: Belongs to the limacoditoxin-3 family. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         18..59
FT                   /note="U-limacoditoxin(3)-Dv32a"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453404"
FT   DISULFID        22..38
FT                   /evidence="ECO:0000305|PubMed:33893140"
FT   DISULFID        29..44
FT                   /evidence="ECO:0000305|PubMed:33893140"
FT   DISULFID        37..53
FT                   /evidence="ECO:0000305|PubMed:33893140"
SQ   SEQUENCE   59 AA;  6624 MW;  5BDC2173E29160E1 CRC64;
     MLRLIMIVTA LILLAAAEPE DCSWPDEHCN LFKTGQCCDR SMSCVVKNWK TVCSAGWFS