ID   U333_DORVU              Reviewed;          59 AA.
AC   P0DUS9;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=U-limacoditoxin(3)-Dv33 {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(3)-Dv33 {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Cecropin-like peptide {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-59, FUNCTION,
RP   SUBCELLULAR LOCATION, RECOMBINANT EXPRESSION, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Probable toxin. Shows a relatively potent antiparasitic
CC       activity against the major pathogenic nematode of ruminants
CC       (H.contortus, EC(50)=2.6 uM). Does not show insecticidal and
CC       antimicrobial activities. Does not induce increase in intracellular
CC       calcium in mouse DRG neurons, suggesting that it does not induce pain.
CC       {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- PTM: The natural peptide is not amidated. The recombinant peptide is
CC       amidated. {ECO:0000305|PubMed:33893140}.
CC   -!- SIMILARITY: Belongs to the limacoditoxin-3 family. {ECO:0000305}.
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DR   SMR; P0DUS9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         20..59
FT                   /note="U-limacoditoxin(3)-Dv33"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453405"
FT   CONFLICT        20
FT                   /note="A -> G (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305|PubMed:33893140"
SQ   SEQUENCE   59 AA;  6588 MW;  A675FC19B7948F1F CRC64;
     MSKVILLCLI FALFACSISA LSKNKNCLKL GQKCNFEKKR CCTGLNCYIS QNKCLPVKL