C74A3_ORYSJ
ID C74A3_ORYSJ Reviewed; 500 AA.
AC Q6Z6L1; Q0E2R7;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Allene oxide synthase 3;
DE EC=4.2.1.92;
DE AltName: Full=Cytochrome P450 74A3;
DE AltName: Full=Hydroperoxide dehydrase 3;
GN Name=CYP74A3; Synonyms=AOS3;
GN OrderedLocusNames=Os02g0218700, LOC_Os02g12680; ORFNames=P0027A02.12;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP TISSUE SPECIFICITY, INDUCTION, AND NOMENCLATURE.
RX PubMed=14988482; DOI=10.1093/pcp/pch025;
RA Haga K., Iino M.;
RT "Phytochrome-mediated transcriptional up-regulation of ALLENE OXIDE
RT SYNTHASE in rice seedlings.";
RL Plant Cell Physiol. 45:119-128(2004).
CC -!- FUNCTION: Involved in the biosynthesis of jasmonic acid, a growth
CC regulator that is implicated also as a signaling molecule in plant
CC defense. Converts 13-hydroperoxylinolenic acid to 12,13-epoxylinolenic
CC acid (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(13S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoate =
CC (9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O;
CC Xref=Rhea:RHEA:25074, ChEBI:CHEBI:15377, ChEBI:CHEBI:36438,
CC ChEBI:CHEBI:58757; EC=4.2.1.92;
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis.
CC -!- TISSUE SPECIFICITY: Not expressed in dark-grown seedlings.
CC {ECO:0000269|PubMed:14988482}.
CC -!- INDUCTION: Not induced by red (R) light, or abrasion in dark-grown
CC seedlings. {ECO:0000269|PubMed:14988482}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AP004996; BAD17182.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08221.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77678.1; -; Genomic_DNA.
DR EMBL; AK107161; BAG97978.1; -; mRNA.
DR RefSeq; XP_015624763.1; XM_015769277.1.
DR AlphaFoldDB; Q6Z6L1; -.
DR SMR; Q6Z6L1; -.
DR STRING; 4530.OS02T0218700-01; -.
DR PaxDb; Q6Z6L1; -.
DR PRIDE; Q6Z6L1; -.
DR EnsemblPlants; Os02t0218700-01; Os02t0218700-01; Os02g0218700.
DR GeneID; 4328742; -.
DR Gramene; Os02t0218700-01; Os02t0218700-01; Os02g0218700.
DR KEGG; osa:4328742; -.
DR eggNOG; ENOG502QQNS; Eukaryota.
DR HOGENOM; CLU_045757_0_0_1; -.
DR InParanoid; Q6Z6L1; -.
DR OMA; HARRDMV; -.
DR OrthoDB; 871849at2759; -.
DR PlantReactome; R-OSA-1119332; Jasmonic acid biosynthesis.
DR UniPathway; UPA00382; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0009978; F:allene oxide synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 2: Evidence at transcript level;
KW Fatty acid biosynthesis; Fatty acid metabolism; Heme; Iron;
KW Lipid biosynthesis; Lipid metabolism; Lyase; Metal-binding;
KW Oxylipin biosynthesis; Reference proteome.
FT CHAIN 1..500
FT /note="Allene oxide synthase 3"
FT /id="PRO_0000052126"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 297..298
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 304
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 365..368
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 452
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 500 AA; 54439 MW; 5472C38A23AAD812 CRC64;
MAPPPVNSGD AAAAATGEKS KLSPSGLPIR EIPGGYGVPF FSPLRDRLDY FYFQGAEEYF
RSRVARHGGA TVLRVNMPPG PFISGNPRVV ALLDARSFRV LLDDSMVDKA DTLDGTYMPS
RALFGGHRPL AFLDAADPRH AKIKRVVMSL AAARMHHVAP AFRAAFAAMF DAVEAGLGAA
VEFNKLNMRY MLDFTCAALF GGEPPSKVVG DGAVTKAMAW LAFQLHPIAS KVVKPWPLEE
LLLHTFSLPP FLVRRGYADL KAYFADAAAA VLDDAEKSHT GIPRDELLDN LVFVAIFNAF
GGFKIFLPHI VKWLARAGPE LHAKLATEVR ATVPTGEDDG ITLAAVERMP LVKSVVWEAL
RMNPPVEFQY GHARRDMVVE SHDAAYEVRK GEMLFGYQPL ATRDEKVFDR AGEFVADRFV
AGGAAGDRPL LEHVVWSNGP ETRAPSEGNK QCPGKDMVVA VGRLMVAELF RRYDTFAADV
VEAPVEPVVT FTSLTRASSG
