U520_DROME
ID U520_DROME Reviewed; 2142 AA.
AC Q9VUV9; Q5U0X1; Q8T9I9;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 4.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Putative U5 small nuclear ribonucleoprotein 200 kDa helicase;
DE EC=3.6.4.13;
DE AltName: Full=Protein lethal (3) 72Ab;
GN Name=l(3)72Ab; ORFNames=CG5931;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1212-2130.
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Putative RNA helicase involved in the second step of RNA
CC splicing. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: Composed of two similar domains.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39415.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF49564.4; -; Genomic_DNA.
DR EMBL; BT016121; AAV37006.1; -; mRNA.
DR EMBL; AY069270; AAL39415.1; ALT_INIT; mRNA.
DR RefSeq; NP_648818.3; NM_140561.4.
DR AlphaFoldDB; Q9VUV9; -.
DR SMR; Q9VUV9; -.
DR BioGRID; 65049; 19.
DR IntAct; Q9VUV9; 7.
DR MINT; Q9VUV9; -.
DR STRING; 7227.FBpp0075282; -.
DR PaxDb; Q9VUV9; -.
DR PRIDE; Q9VUV9; -.
DR DNASU; 39737; -.
DR EnsemblMetazoa; FBtr0075527; FBpp0075282; FBgn0263599.
DR GeneID; 39737; -.
DR KEGG; dme:Dmel_CG5931; -.
DR FlyBase; FBgn0263599; l(3)72Ab.
DR VEuPathDB; VectorBase:FBgn0263599; -.
DR eggNOG; KOG0951; Eukaryota.
DR GeneTree; ENSGT00940000154966; -.
DR HOGENOM; CLU_000335_2_1_1; -.
DR InParanoid; Q9VUV9; -.
DR OMA; ESFWIIV; -.
DR OrthoDB; 154891at2759; -.
DR PhylomeDB; Q9VUV9; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway.
DR SignaLink; Q9VUV9; -.
DR BioGRID-ORCS; 39737; 0 hits in 1 CRISPR screen.
DR ChiTaRS; l(3)72Ab; fly.
DR GenomeRNAi; 39737; -.
DR PRO; PR:Q9VUV9; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0263599; Expressed in eye disc (Drosophila) and 25 other tissues.
DR Genevisible; Q9VUV9; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0030532; C:small nuclear ribonucleoprotein complex; IPI:FlyBase.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR GO; GO:0000388; P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11); IBA:GO_Central.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.300; -; 4.
DR InterPro; IPR041094; Brr2_helicase_PWI.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 2.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF18149; Helicase_PWI; 1.
DR Pfam; PF02889; Sec63; 2.
DR SMART; SM00487; DEXDc; 2.
DR SMART; SM00490; HELICc; 2.
DR SMART; SM00973; Sec63; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
DR SUPFAM; SSF52540; SSF52540; 4.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR PROSITE; PS51194; HELICASE_CTER; 2.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW Nucleotide-binding; Nucleus; Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..2142
FT /note="Putative U5 small nuclear ribonucleoprotein 200 kDa
FT helicase"
FT /id="PRO_0000102089"
FT DOMAIN 490..673
FT /note="Helicase ATP-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 684..917
FT /note="Helicase C-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 981..1286
FT /note="SEC63 1"
FT DOMAIN 1337..1511
FT /note="Helicase ATP-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 1544..1752
FT /note="Helicase C-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 1811..2128
FT /note="SEC63 2"
FT REGION 52..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..396
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 615..618
FT /note="DEIH box"
FT MOTIF 1453..1456
FT /note="DELQ box"
FT COMPBIAS 368..392
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 503..510
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT BINDING 1350..1357
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 2142 AA; 244510 MW; 10CFE9E84438F2BE CRC64;
MADAAARQLQ YEYKANSNLV LQADVRLIER PRRDEATGEV CSLVGKLDGT RMGDRYQRTK
PEKTEERKVK RQKRDEAQYD FERMKGATLL SEGIDEMVGI VYRPKTQETR QTYEVLLSFI
QEALGDQPRD ILCGAADEIL AVLKNDRLKD RERKKDIDSL LGAVTDERFA LLVNLGKKIT
DFGSDAVNAL TAAPNNEEQI DETYGINVQF EESEEESDND MYGEIRDDDA QDEGEEARID
HTLHAENLAN EEAANNVKKE RSLHPLDIDA YWLQRCLSKF YKDAMVSQSK AADVLKILKD
AADDRDCENQ LVLLLGYDCF DFIKQLKLNR QMVLYCTMLA SAQTDSERQR IREKMRGNSA
LAKILRQLDT GKSEDQEEGE ARGSKRGKGD AEDGGAAAAG QVAGVRQLLE LEEMAFTQGS
HFMANKRCQL PDGSYRKQRK GYEEVHVPAL KPVPFDANEE LQPVDKLPKY VQPVFEGFKT
LNRIQSRLYK AALDSDENML LCAPTGAGKT NVALLTMMRE IGKHINEDGT INAQDFKIIY
VAPMKSLVQE MVGNFGRRLA CYNLTVSELT GDHQLTREQI AATQVIVCTP EKWDIITRKG
GERTFVSLVR LVIIDEIHLL HDERGPVLEA LVARTIRNIE TTQEEVRLVG LSATLPNYQD
VATFLRVKPD KGLFYFDNSY RPVSLEQQYI GVTEKKALKR FQVMNEIVYE KTMEHAGRNQ
VLVFVHSRKE TGKTARAVRD MCLEQDTLGS FLKEGSASME VLRTEAEQVK NTELKELLPY
GFAIHHAGMT RVDRTLVEDL FADRHIQVLV STATLAWGVN LPAHTVIIKG TQVYNPEKGR
WVELSALDVL QMLGRAGRPQ YDTKGEGILI TNHSELQFYL SLLNQQLPIE SQFISKLPDM
LNAEIVLGTV QHLQDAVNWL GYTYLYIRML RNPTLYGVSH DAIKADPLLE QHRADLLHTA
ACCLERSGLI KYDRKTGHFQ VTDLGRIASH YYLTHETMLT YNQLLKQTLS EIELFRVFSL
SSEFRHISVR EEEKLELQKL MERVPIPIKE SIEEHSAKVN VLLQAYISQL KLEGFALMSD
MVFITQSAAR LMRAIFEIVL TRGWAQLADK TLTLCKMIDR RMWQSMTPLR QFKKMPDEIA
KKLEKKHFPW GRLYDLEPHE LGELIRVPKL GKTIHKFVHQ FPKLELSTHI QPITRGTLRV
ELTITPDFQW DEKVHGQSEG FWVLIEDVDS ELILHHEFFL LKQKYSQDEH QLKFFVPVFE
PLPPQYFLRI VSDRWIGAET QLPVSFRHLI LPEKNMPPTE LLDLQPLPIS ALRQPKFESF
YSQRFPQFNP IQTQVFNAVY NSDENVFVGA PTGSGKMTIA EFAIMRLFTT QSDARCVYLV
SEEALADLVF ADWHSKFGSL DIKVVKLTGE TGTDLKLIAK GQLVITTADK WDVLSRRWKQ
RKNVQLVNLF IVDELQLVGG EEGPVLEIVC SRMRYISSQI EKQIRIVALS ASLTDARDVA
QWLGCNPNAT FNFHPSVRPI PLELHIQGYN VTHNATRIAT MSKPVYNAIL KYSAHKPVIV
FVSSRKQARL TAIDVLTYAA SDLQPNRFFH AEEEDIKPFL ERMTDKTLKE TLAQGVAYLH
EGLSASDHRL VEQLFDSGAV QVAVISRDLC WGMSISAHLV IIMDTQFYNG KNHSYEDYPI
TDVLQMIGRA NRPNEDADAK CVLMCQSSKK DFFKKFINEP LPIESHLDHR MHDHFNAEVV
TKTIENKQDA VDYLTWTFLY RRLTQNPNYY NLQGVTHRHL SDHLSELVEN TLSDLEQSKC
ISVEDDMDTL PLNLGMIAAY YYINYTTIEL FSLSLNSKTK VRGLLEIISS AAEYEDVVVR
HHEEQVLRTL SQRLPNKLTG PNETAPKFND PHIKTNLLLQ AHLSRLQLGP ELQGDTEQIL
SKAIRLIQAC VDVLSSNGWL SPAVAAMELA QMVTQAMWSK DSYLKQLPHF SPEIVKRCTE
KKIETVFDIM ELEDEDRTRL LQLSDLQMAD VARFCNRYPN IELNYEVVDK DRINSGSTVN
VVVQLEREDE VTGPVIAPFF PQKREEGWWV VIGDPKTNSL LSIKRLTLQQ KAKVKLDFVA
PSPGKHDYTL YYMSDSYLGC DQEYKFSIEV GDFQSESESE SD
